Nanomed. J. 6(2): 128-135, Spring 2019 RESEARCH PAPER Comparison of the efects of silver nanoparticles and silver cobalt nanoparticles on function tests and liver tissue changes in adult male rats Zohreh Parang 1* , Mojdeh Parsaeian 1 , Davood Moghadamnia 2 1 Department of Physics, Shiraz Branch, Islamic Azad University, Shiraz, Iran 2 Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University, Shiraz, Iran * Corresponding Author Email: zohreh.parang@gmail.com Note. Tis manuscript was submitted on November 11, 2018; approved on February 25, 2019 How to cite this article Parang Z, Parsaeian M, Moghadamnia D. Comparison of the efects of silver nanoparticles and silver cobalt nanoparticles on function tests and liver tissue changes in adult male rats. Nanomed J. 2019; 6(2): 128-135. DOI: 10.22038/nmj.2019.06.0007 ABSTRACT Objective(s): Tis study aimed to compare impacts of silver nanoparticles and silver cobalt nanoparticles on the hepatic function tests and changes in liver tissues in adult male rats. Materials and Methods: Tis study was conducted on 49 adult male Wistar rats, each weighing approximately 180-220 gr. Te rats were randomly assigned to seven groups of seven including one control group and six experimental groups. Te experimental groups 1 and 2 respectively received 25 and 100 mg/kg of silver nanoparticles synthesized for 75 sec intraperitoneally for 14 days. Experimental group 3 received silver nanoparticles that were synthesized at 300 sec which were administered intraperitoneally in a 25 mg/kg dose for 14 days. Te experimental groups 4 and 5 received silver cobalt nanoparticles, whereby silver nanoparticles were synthesized at 75 sec and were administered intraperitoneally in a 25 and 100 mg/ kg dose for 14 days, respectively. Finally, experimental group 6 received a 25 mg/kg dose of silver cobalt nanoparticles, intraperitoneally for 14 days, with the silver nanoparticles synthesized for 300 sec. At the end of this period, the serum levels of hepatic enzymes, albumin, and total protein were measured and tissue changes were evaluated in this study. Results: Te mean serum levels of AST, total protein, and albumin in the experimental groups 1 and 3 increased signifcantly compared to the control group. Similarly, the mean serum levels of ALT and ALP in the experimental group 3 showed a signifcant increase in comparison with the control group. Te mean of liver weight in all experimental groups was signifcantly higher than the control group(P<0.05). Furthermore, the necrosis of the liver tissue was observed in the recipients of silver nanoparticles. However, liver necrosis was not observed in the groups receiving silver cobalt nanoparticles. Conclusion: Te use of silver nanoparticles can boost the serum levels of hepatic enzymes and increase liver tissue necrosis, as well. However, the silver cobalt nanoparticles did not change the levels of hepatic enzymes and liver tissue. Keywords: Albumin, Hepatic Enzyme, Rat, Silver Nanoparticles, Silver Cobalt Nanoparticles INTRODUCTION There is a growing increase of the applicaton of nanotechnology products in human actvity. Silver nanopartcles (Ag NPs ) are nanopartcles of silver with the size of 1-100 nanometer [1]. The efects of silver nanopartcle poisoning on none-mammalian species, cell lining, and bacteria have been proven in the literature [2]. Studies of in vitro have shown that poisoning with silver nanopartcles includes reactve oxygen species producton with oxidatve stress, interacton with enzymes and proteins to free thiol group, and the mimic of endogenous ions of calcium, sodium, and potassium. These mechanisms result in cytokine producton, cellular damage, and ultmately cell death and necrosis.