Seminar Nasional Riset Kedokteran (SENSORIK) 2020 287 UJI EFEKTIVITAS EKSTRAK DAUN PACAR AIR (Impatiens balsamina) TERHADAP MORTALITAS LARVA Aedes aegypti Finy Widyanti Hadi 1* , Yuni Setyaningsih 2** , dan Mila Citrawati 3 1 Program Studi Kedokteran Program Sarjana, Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia 2 Departemen Parasitologi, Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia 3 Departemen Fisiologi, Fakultas Kedokteran, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia *finywidyantihadi@gmail.com **yunisty00@gmail.com Abstract. Natural larvicide can be used as a new alternative to shyntetic larvicide to control the Dengue Hemorrhagic Fever (DHF) vector, one of which was pacar air leaf ( Impatiens balsamina). Phytochemical content of pacar air leaves were Alkaloids, Saponins, Tannins, Flavanoids, and Steroids which were secondary metabolites and larvicidal compounds. This study aimed to determine the effect of pacar air leaf extract as Aedes aegypti larvicide. The design of this research was experimental which contains 6 treatment groups, consisting of; negative control (100 ml aquades), positive control (abate 1%), and the treatment group with variant concentrations (0.25%; 0.50%; 0.75%; 1%). Each group contained 25 larvae in 100 ml extract solution with 4 repetitions. The test used was the one-way ANOVA test and the Bonferroni post-hoc test. The result of the one-way ANOVA test showed p value of 0.00 and the post-hoc Bonferroni test that there was no difference between concentrations of 1% with positive control, while there was a difference between concentrations of 0.25%; 0.50% and 0.75% with negative controls. That was because the content of secondary metabolites in pacar air leaves that works simultaneously kill the larvae with highest larva mortality at a concentrations of 1%. Keywords: Aedes aegypti, Larvaside, Pacar air leaf (Impatiens balsamina) 1. Pendahuluan Di Indonesia penyakit Demam Berdarah Dengue (DBD) masih merupakan masalah kesehatan dengan jumlah kasus yang cenderung meningkat dari tahun ke tahun. Indonesia merupakan salah satu negara endemik Demam Dengue yang setiap tahunnya selalu terjadi KLB di berbagai kota dan setiap lima tahun sekali terjadi KLB besar. Meningkatnya angka kejadian Demam Berdarah di Indonesia akibat sulitnya pengendalian penyakit yang ditularkan oleh nyamuk Aedes aegypti [1] . Berdasarkan data yang diambil dari Direktorat Pencegahan dan Pengendalian Penyakit Tular Vektor dan Zoonotik, pada tahun 2015 didapatkan sebanyak 129.560 penderita dengan 1.071 di