Int.J.Curr.Microbiol.App.Sci (2019) 8(3): 851-860 851 Original Research Article https://doi.org/10.20546/ijcmas.2019.803.102 Effect of Epigenetic Modifiers on Fermentation Parameters of Endophytic Fungi from Plants Growing in Uzbekistan T.G. Gulyamova, L.I. Abdulmyanova, D.M. Ruzieva, G.A. Rasulova, U.K. Yusupov and R.S. Sattarova * Department of Biochemistry and Biotechnology of Physiologically Active Compounds, Institute of Microbiology of the Academy of Sciences RU, Uzbekistan *Corresponding author ABSTRACT Introduction Endophytic fungi produce a variety of bioactive compounds of various chemical classes, including alkaloids, terpenoids, flavonoids, quinones, steroids, phenolic acids, etc., exhibiting antimicrobial, anticancer, immunomodulatory activities (Zhang et al., 2006; Nisa et al., 2015). In particular, a plethora of different endophytes is reported to produce most of the in-demand bioactive molecules including vincristine, vinblastine, camptothecin, huperzine, podophyllotoxin, diosgenin and azadirachtin (Zhao et al., 2011b). However, the potential of endophytes as sustainable alternative production platforms is hampered due to the substantial reduction in secondary metabolite production upon repeated subculturing under axenic monoculture conditions. As possible reasons for the attenuation lack of host stimuli in culture medium and genomic instability or silencing of the biosynthetic genes in axenic cultures have been hypothesized (Cichewicz et al., 2010). The present study aimed to investigate the effects of addition of epigenetic modifiers in the growth medium on biomass and secondary metabolites production in the suspension cultures of the endophytes Thielavia microspora MO46L with antimicrobial activity, isolated from Melissa officinalis, Penicillium concavoradulozum VE89L and Aspergillus amstelodami VR177L isolated from Vinca plants, both producing vinblastine and vincristine. The obtained data showed that quercetin and nicotinamide affected the physiological and cultural properties of studied endophytic strains as well as their metabolic profile. Results of this study also showed that quercetin can be used to induce the synthesis of vinblastine as target product in the endophytic fungi P. concavoradulozum VE89L and A. amstelodami VR177L. Keywords Endophytic fungi, Secondary metabolites, Epi genetic modifiers, nicotinamide, Quercetin Accepted: 10 February 2019 Available Online: 10 March 2019 Article Info International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 03 (2019) Journal homepage: http://www.ijcmas.com