Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Mon, 03 Dec 2018 21:52:56 Saccharothrix isguenensis sp. nov., an actinobacterium isolated from desert soil Khaoula Bouznada, 1 Noureddine Bouras, 1,2 Salim Mokrane, 1 Fawzia Chaabane Chaouch, 1 Abdelghani Zitouni, 1 Gabriele Pötter, 3 Cathrin Spröer, 3 Hans-Peter Klenk 4 and Nasserdine Sabaou 1 Correspondence Hans-Peter Klenk hans-peter.klenk@newcastle. ac.uk or hans-peter.klenk@ncl.ac.uk Nasserdine Sabaou sabaou@yahoo.fr 1 Laboratoire de Biologie des Syst  emes Microbiens (LBSM), Ecole Normale Sup erieure de Kouba, Algiers, Algeria 2 D epartement de Biologie, Facult e des Sciences de la Nature et de la Vie et Sciences de la Terre, Universit e de Ghardaïa, BP 455, Ghardaïa 47000, Algeria 3 DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany 4 School of Biology, Newcastle University, Ridley Building, Newcastle upon Tyne NE1 7RU, UK A novel actinobacterial strain, designated MB27 T , was isolated from a Saharan soil sample collected in Mzab region (Ghardaïa province, South Algeria). Strain MB27 T was characterized following a polyphasic taxonomic approach. This strain produced a branched and fragmented substrate mycelium, which was found to have a yellowish orange colour. A white scanty aerial mycelium was produced on most media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB27 T belongs to the family Pseudonocardiaceae and is closely related to the genus Saccharothrix. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose, ribose and small amounts of mannose and rhamnose. The detected phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Mycolic acids were not detected while the predominant fatty acid was iso-branched hexadecanoate (iso-C 16 : 0 ). The major menaquinone was MK-9(H 4 ). Results of 16S rRNA gene sequence comparisons revealed that strain MB27 T shairs the highest degree of similarity with Saccharothrix ecbatanensis DSM 45486 T (99.8%), Saccharothrix hoggarensis DSM 45457 T (99.3 %), Saccharothrix longispora DSM 43749 T (98.6 %) and Saccharothrix yanglingensis DSM 45665 T (98.6 %). However, it exhibited only 11- 42 % DNA–DNA relatedness to the neighbouring Saccharothrix species. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA–DNA hybridization, strain MB27 T is shown to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix isguenensis sp. nov. (type strain MB27 T =DSM 46885 T =CECT 9045 T ) is proposed. The genus Saccharothrix was first described by Labeda et al. (1984), with Saccharothrix australiensis as the type species. This genus, which was subsequently classified in the family Pseudonocardiaceae (Labeda et al., 2011), is characterized by fragmentation of both substrate and aerial mycelia into non-motile rods and ovoid elements. This genus is also characterized by a type III cell wall containing meso-diami- nopimelic acid (meso-DAP) without glycine, galactose, rhamnose and small amounts of mannose as diagnostic whole-cell sugars, a phospholipid type PII (phosphatidyl- ethanolamine) or PIV (phosphatidylethanolamine and phospholipids containing glucosamine) pattern (Labeda & Lechevalier, 1989), and MK-9(H 4 ) as the predominant menaquinone, while mycolic acids are absent (Labeda & Kroppenstedt, 2000). However, the accumulated chemotax- onomic and phylogenetic data have enabled an intense taxo- nomic reclassification of the genus Saccharothrix, with several strains transferred to other new taxa, including the genera Lentzea (Yassin et al., 1995), Crossiella (Labeda, Abbreviation: DAP, diaminopimelic acid. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MB27 T is KU933253. Two supplementary figures and one supplementary table are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2016), 66, 4785–4790 DOI 10.1099/ijsem.0.001430 001430 ã 2016 IUMS Printed in Great Britain 4785