©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 12561-12566 (2015) Differentiated evolutionary relationships among chordates from comparative alignments of multiple sequences of MyoD and MyoG myogenic regulatory factors L.C. Oliani 1 , K.C.F. Lidani 2 and J.E. Gabriel 1 1 Colegiado de Ciências Biológicas, Campus de Ciências Agrárias, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brasil 2 Departamento de Patologia Médica, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brasil Corresponding author: J.E. Gabriel E-mail: jane.gabriel@univasf.edu.br Genet. Mol. Res. 14 (4): 12561-12566 (2015) Received May 27, 2015 Accepted August 13, 2015 Published October 16, 2015 DOI http://dx.doi.org/10.4238/2015.October.16.23 ABSTRACT. MyoD and MyoG are transcription factors that have essential roles in myogenic lineage determination and muscle differentiation. The purpose of this study was to compare multiple amino acid sequences of myogenic regulatory proteins to infer evolutionary relationships among chordates. Protein sequences from Mus musculus (P10085 and P12979), human Homo sapiens (P15172 and P15173), bovine Bos taurus (Q7YS82 and Q7YS81), wild pig Sus scrofa (P49811 and P49812), quail Coturnix coturnix (P21572 and P34060), chicken Gallus gallus (P16075 and P17920), rat Rattus norvegicus (Q02346 and P20428), domestic water buffalo Bubalus bubalis (D2SP11 and A7L034), and sheep Ovis aries (Q90477 and D3YKV7) were searched from a non-redundant protein sequence database UniProtKB/Swiss-Prot, and subsequently analyzed using the Mega6.0 software. MyoD evolutionary analyses revealed the presence of three main clusters with all mammals branched in one cluster,