65 Podium Sessions Combined treatments IP• Immunohistochemical expression of c-erbB family gene products in salivary gland malignant neoplasms: a possible role for immunotherapy A.K. Poulopoulos 1, A. Kmridou ~, K. Vahtsevanos 3, A. Epivat~anos I, K. Antomades 4, D. Antomades 1 . ~Dept of Oral Me&cme & Pathology. Dental School. Artstotle Untvers~ty of Thessalomkz. Greece. 2Dept o f Htstopatholog3: Yheagemon Ant~cancer Hospttal, ThessaIontl~, Greece, SDept of Oral & MaxzIlofactaI Surgeo', Theagenton Anttcancer Hospttal, ThessaIantk~, Greece, 4Dept o f Oral & MaxtIlofactal Surgery Dental School, Artstotle Untvers~ty of Thessalontla. Greece Introduction: Sallvary gland cancers are a rare mahgnancy accoan~tang for less than 1% of all cancers and 3-6% of cancers of the head and neck region. The classaficatlon of sahvary gland tumors is traditionally based on morphology and the dafferent subtypes exhibit various chnlcal behavaors. The tyrosane kanase receptor fannly, including c-erbB2 has been recognized as beang of partacular anlportance an many hurnan mahgnancles, such as breast cancer. Thas study was undertaken to define the role of c-erbB2 an sahvary gland mahgnant neoplasms. Materials and Methods: The study cm~sasted of 33 samples of formalan-fixeck paraffin-embedded specunens of sahvary gland mucoepldermoad carcanomas, adenoid cystac carcinomas and adenocarcanomas. 8pecan~ens were staaned with mouse antfllmnan monoclonal antabody for ammunotnstochermcal de- tectaon of c-erbB2. Overlyang oral mucosa and adjacent normal sahvary ducts served as internal controls. Light mlcrosco W evaluation of anwnananstaaned sections was performed, by scor- aug the percentage of positively stained ~nor cells mad the antensity of the staanmg. Results: Posabve unmunostamang for biomarker c-erbB2 was observed an the cytoplasm and membrane o f all tumors. Among the tumors c-erbB2 was strongly expressed in adenoid cystic carcinomas followed by adenocarcanomas, and high grade mucoepadermo ld carclnoanas. Conclusion: One of the most anterestang therapies that has been used an breast cancer is trastuzunmd, an unrntmotherapy based treatment, an patients whose spectmens overexpress c-erbB2 protean. The overexpression of the c-erbB2 oncoprotean an sahvary gland tumors has been associated with blologmal aggresmveness and poor prognosis an most cases. Given the suboptanaal response rates, duralaon of response, and toxicity of conventional chemotherapy, a better understanding of the banlogy of sahvary gland mahgnancies will lead to tmproved prognostacation and treatment. With the emergence of molecu- lar targeted therapy, these tumors become an optanlal canchdate for trials of new drugs such as trastuzumab (Herceptan), and estabhshed dugs for new andmahons. PD.21 TIP (taxanes, ifosphamide, and platinum): induction therapy in locally advanced head and neck cancers S.K. Bishnoi, H.S. Kumar, S. Jain, A. Kalwar, A. Sharma, M.R. Bardaa, D.E Puma. Acharya Tulsi Regional Cancer Treatment and Research Institute, Bzkaner (RajasthatO, India Introduction: Head and neck cancer are most common ma- hgnancies an Inchan subcontanent. Most of the patient presents an advanced stage and rachotherapy is the still the prancipal treatment modahty, h~luction chemotherapy is appropriate for the treatment of locally advanced squmaous cell carcanoma of head and neck because it downstage the tmnor prior to rachotherapy, allows organ preservation without compromlsang the SUl~-aval. TIP protocol is chosen because ~t has shown su- perior complete response rate with possible surwval advantage an advanced nasopharyngeal carcanomas. Materials and Methods: 108 cases of locally advanced head and neck cancer, stage 111 / iv treated with 3 cycle of TIP (pachtaxel-175mg/M2 day 1, Ifospharmde-l.2gm/M2 dayl 3 and cisplatln-100 m~M2 divided over 3 days) anduction ther- apy followed by External beam rachotherapy 60-64 Gy by 2Gy per fraction, 5 days a week over 6 weeks, at our institute be- tween october2000 and sept2004, all the patients were analyzed for response and acute toxacaty. Results: Out of 108, 102 were male. The mechan age was 56 years. According to the primary site of the lesion- base of tongue (23°,'o), oral cavity (25°,'o), laryngopharynx (20%) tonsil (14%) and others (18%). The enttre pahent completed 3 cycle of chemotherapy without any mortahty. Overall response at the prunary tumors sate was observed in 92patients (85%), CR in 70patients (65%) and PR in 22(20%). At lymph node sates 76 patients (7026) showed response, CR-45%, and PR-25%. Survival data not yet analyzed. Amongst the toxicity 4Jr an/iv neutropenia m 30% cases with febrile neutropema m 5%. Conclusion: TIP protocol is well tolerated regtmens, pro- duces higher response rate an locally advanced head and neck sqmnaous cell carcinoma. The effect on the survival is not yet known. [•1 Oral pilocarpine as preventive treatment for radiation-induced xerostomia. Preliminary report K. Luna-Ortiz I, A. Mosqueda-Taylor 2, M.E. Irigoyen- Camacho 2, A. Poltevm-Chacon 3, M.A. Diaz Franco 2, M. Rascdn-Ortiz I , M. Granados-Garcia I, J.L. Agmlar-Ponce 4, A.M. Coll-Mufioz 5. 1 Departamento de Czrurgia de Cabeza y Cuello, Mexzco; 2 Departamento de Atenc~bn a la Salud Un~vers~dad Autonoma Metropohtana XoclumHco. Mexzeo; 3 Departamento de Rad~oterapm, Mexico; 4 Departamento de Oncologia Mddtca Instttuto Naczonal de Cancerologia. Mexico, 5 Monitor clinico Laboratortos Lemery S A de C V. Mexico Introduction: Irreversible xerostorma is one of the most fre- quent and slgmficant radmtion-anduced side effects m patients treated of head and neck mahgnaacles. Prevenhon of tins comphcatmn is usually dafficult to achieve because salivary glands are within the radiation field m most cases. Recent