COMBINED HUMAN AND PORCINE MASS CHEMOTHERAPY FOR THE CONTROL OF T. SOLIUM H. H. GARCIA,* A. E. GONZALEZ, R. H. GILMAN, L. H. MOULTON, M. VERASTEGUI, S. RODRIGUEZ, C. GAVIDIA, V. C. W. TSANG, AND THE CYSTICERCOSIS WORKING GROUP IN PERU Department of Microbiology, Universidad Peruana Cayetano Heredia, San Martin de Porras, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurologicas, Barrios Altos, Lima, Peru; Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Salamanca de Monterrico, Lima, Peru; A.B.PRISMA, San Miguel, Lima, Peru; Parasitic Diseases Branch, Centers for Disease Control, Atlanta, Georgia Abstract. A combined (human and porcine) mass chemotherapy program was tested in a controlled design in 12 village hamlets in the Peruvian highlands. A single dose of 5 mg of praziquantel was given to eliminate intestinal taeniasis in humans, and two rounds of oxfendazole (30 mg/kg) were administered to all pigs. The total population in the study villages was 5,658 resident individuals, and the porcine population at the beginning of the study was 716 pigs. Human treatment coverage was 75%, ranging from 69% to 80%. There were only a few refusals of owners for porcine treatment of their animals. The effect of the intervention was measured by comparing incidence rates (seroconversion in pigs who were seronegative 4 months before) in treatment versus control villages, before and up to 18 months after treatment. There was a clear effect in decreasing prevalence (odds ratio, 0.51; P < 0.001) and incidence (odds ratio, 0.39; P 0.013) in the treatment area after the intervention, which did not leave to extinction of the parasite but stabilized in slightly decreased rates persisting along the follow-up period. Mass chemotherapy was effective in decreasing infection pressure in this hyperendemic area. However, the magnitude of the effect was small and did not attain the goal of eliminating transmission. INTRODUCTION Taenia solium taeniasis/cysticercosis is endemic in most de- veloping countries. 1,2 The adult tapeworm (taeniasis) occurs only in humans and carries mild clinical manifestations or none at all. However, it is the source of infection with the larval form (cysticercosis), which affects humans and pigs. Human cysticercosis is a major cause of seizures and other neurologic symptoms in disease-endemic zones, 3–6 and por- cine cysticercosis carries important economical losses caused by damaged pork. 1,7 The life cycle is sustained because of domestic pig raising and lack of sanitary conditions, which permit free-ranging pigs access to contaminated feces from a tapeworm carrier. T. solium infection has been included into a short list of eradicable parasitic diseases, 8,9 on the basis of several factors: 1) evidence of eradication in defined geographic areas (Eu- rope, North America) through improvements in sanitation and living conditions; 2) a single (human) definitive host, source of cysticercosis infection; 3) domestic animals perpetu- ating the cycle, wild cycle not important in transmission; and 4) availability of control measures, including massive anthel- minthic treatment of humans in endemic areas. 10,11 Currently, control of porcine cysticercosis is based on inspection and condemnation of infected pig carcasses. 12 Less than 10% of Peruvian pigs, however, are registered, and 55% are illegally slaughtered. 7 In abattoirs where control and confiscation are not carried out, rates of infection among pigs may be as high as 30%. 7 Thus, control measures to prevent human consump- tion are impractical and currently inadequate in endemic ar- eas. A series of specific control measures, in addition to abattoir inspection, must be instituted to prevent human and swine cysticercosis. 13 Several groups have attempted to actively control T. solium in endemic areas by applying mass human chemotherapy, 14–18 pig immunization, 19–22 health educa- tion, 23 and other interventions. 24 However, elimination of taeniasis/cysticercosis has not been achieved to date, the ef- fect of interventions has always been partial, and no conclu- sive data on sustainability exist. From all attempted interven- tions, mass chemotherapy has been consistently shown to de- crease prevalence of infection in Ecuador, 14 Mexico, 17 and Guatemala, 18 and thus seemed to be the most promising tool. By adding the use of oxfendazole, recently described to be effective for porcine cysticercosis, 25 we performed, in an en- demic area of Peru, the first combined human and porcine mass chemotherapy trial to control the transmission of T. solium. MATERIALS AND METHODS Overall study design. Cohort study to evaluate the effect of a combined (human and porcine chemotherapy) intervention for the control of T. solium comparing the prevalence and incidence of porcine cysticercosis in treated versus compari- son areas. Outcomes were evaluated every 4 months after the intervention for a total of 20 months. Study site. The central highland area of Peru was selected because of shown endemicity 7 and accessibility by road. The zone is cold and has at least two clearly defined seasons: dry from June to November and wet from December to May. Paved access highways are passable during all of the year, although connecting roads may not be used during the rainy season. A preliminary trip was performed to select an ad- equate study area on the basis of accessibility and their will- ingness to cooperate in a longitudinal project on the control of porcine cysticercosis. In this trip, several villages located less than 50 miles from Huancayo (population 300,000), the main city in the Department, were visited and inspected. The selection of communities near Huancayo was made because of the convenience of a city-based location for specimen han- dling, centrifugation, and storage. * Address correspondence to Hector H. Garcia, Department of Mi- crobiology, Universidad Peruana Cayetano Heredia. Av. H. Delgado 430, SMP, Lima 31, Peru. E-mail: hgarcia@jhsph.edu Am. J. Trop. Med. Hyg., 74(5), 2006, pp. 850–855 Copyright © 2006 by The American Society of Tropical Medicine and Hygiene 850