~ 1368 ~ Journal of Pharmacognosy and Phytochemistry 2020; 9(4): 1368-1370 E-ISSN: 2278-4136 P-ISSN: 2349-8234 www.phytojournal.com JPP 2020; 9(4): 1368-1370 Received: 04-05-2020 Accepted: 06-06-2020 Alle Rakesh MSc Scholar, Uttaranchal University, Department of Plant Pathology, SOA, Bhubaneswar, Odisha, India Jai Prakash Mishra Principal & Professor, Department of Plant Pathology, Uttaranchal University, School of Agriculture, Dehradun, Uttarakhand, India. Rajendra Prasad Assistant Professor, Department of Plant Pathology, Uttaranchal University, School of Agriculture, Dehradun, Uttarakhand, India. J Chandra Sekhar Uttaranchal University, School of Agriculture, Department of Plant Pathology, Dehradun, Uttarakhand, India Dharmesh Gupta Principal Scientist, Department of Plant Pathology, Dr.Y.S Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India Vedukola Pulla Reddy Department of Plant Pathology, Dr.Y.S Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India Sunil Kumar Department of Plant Pathology, Dr.Y.S Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, 173230, India Corresponding Author: Alle Rakesh MSc Scholar, Uttaranchal University, Department of Plant Pathology, SOA, Bhubaneswar, Odisha, India Isolation and in vitro evaluation of fungicides against Pestalotiopsis mangiferae causing grey blight of mango Alle Rakesh, Jai Prakash Mishra, Rajendra Prasad, J Chandra Sekhar, Dharmesh Gupta, Vedukola Pulla Reddy and Sunil Kumar Abstract The present investigation entitled “Isolation and in vitro evaluation of fungicides against Pestalotiopsis mangiferae causing grey blight of mango” was under taken to evaluate for various management studies of the given test fungus. The diseased samples were collected from different localities of Uttarkhandi.e Dehradun, Haridwar and Rishikesh during the 2019-20. The pathogen was isolated from the diseased samples and the pathogen was maintained in potato dextrose agar. The potential species of Pestalotiopsis mangiferae was identified and isolated. In vitro evaluation of fungicides were tested. The data regarding the growth inhibition by three fungicides in which (viz., two systemic and one non-systemic) are as follows, among Systemic fungicides were found more inhibitory as compared to non-systemic fungicides even at equal levels of concentration against the pathogen. The data regarding the growth inhibition by two systemic and one non-systemic are as follows, among the systemic fungicide, Carbendazim and Mancozeb shows high efficacious, copper oxychloride has poor inhibiting capacity. Keywords: Modified wakimoto ' s Agar (MWA), rifampicin Introduction Mango (Mangiferaindica L.) is the most important commercially grown fruit crop of the country. Mango is low in calories yet high in nutrients particularly vitamin C, which aids immunity, iron absorption and growth. Mango is a good source of several B vitamins, as well as vitamins A, C, K and E which all helps to boost immunity. In some parts of the world, mango is called “king of fruits”. It’s a drupe or stone fruit, which means that it has a large seed in the middle. India ranks first among the world’s mango producing countries include China, Thailand, Mexico, Pakistan etc. India’s share of production in all over the world is 52%. There are hundreds of types of mango, each with a unique taste, shape, size and colour. India with 24,71,000 acres of mangos (70%of its fruit-growing area) produces 65% of the world’s mango crop 99,20,700 tons. In 1985, mango growers around Hyderabad sought government protection against terrorists who cut down mango orchards unless the owners paid ransom (50,000 rupees in one case). India far outranks all other countries as an exporter of processed mangos, shipping 2/3 of the total 22,046 tons. India in volume of exports are Thailand, 7,74,365 tons, Pakistan and Bangladesh followed by brazil. Mexico ranks 5 th with about 1,00,800 acres and an annual yield of approximately 6,40,000 tons. The Philippines have risen to 6 th place. Tanzania is 7 th , the Dominican Republic 8 th and Colombia 9 th place. Egypt produces 1,10,230 tons of mangos annually and exports moderate amounts to 20 countries in the near East and Europe. Mango culture in the Sudan occupies about 24,710 acres producing a total of 66,138 tons per year. Materials and Methods Isolation of Pestalotiopsis mangiferae causing grey blight of mango In the present investigation the diseases samples were collected from the field. Small tissues from infected stem or roots (5mm) along with the healthy tissue were cut with sterile scalpel. The tissues were surface sterilized with 0.1 % mercury chloride for 30 seconds. The tissues were subsequently washed in three changes of sterile distilled water to eliminate mercury ions. The surface sterilized tissues were transferred on to the PDA and incubated at 25 ± 2°C in BOD incubator and growth was observed periodically. In-Vitro evaluation of fungicides against pestalotiopsis mangiferae causing grey blight of mango The relative efficacy of three fungicides including systemic and non-systemic fungicides were evaluated under in vitro conditions at three different concentration levels i.e.