129 genes. This report it sets the bases to understand the induction and repression mechanisms of the insect genes, when a GV infection occurs.. Poster / Viruses. Wednesday, 16:30. VI-9 Recombinant Iridovirus IIV-6 expresing the Cn-10 neurotoxin from Centruroides noxius scorpion Flor C. Arellano-Villagómez 1 ; Jorge E. Ibarra 2 ; M. Cristina Del Rincón-Castro 1 1 Food Department, Division of Life Sciences, University of Guanajuato, Irapuato, Gto. México. 2. CINVESTAV-IPN Unidad Irapuato, Irapuato, Gto. México Address for Correspondence: cdelrincon@ugto.mx Was established the methodology to obtain a recombinant Iridovirus in vivo, using the microparticle bombardment. Genes encoding for the green fluorescent protein GFP, and the protein Cn10, was cloned into the 295L gene Iridovirus, strain IIV -6. Were standardized optimal conditions for micro-projectile bombardment cotransfection of the vector DNA TOPO-295L- GFP- Cn10 and wild DNA from Iridovirus IIV-6, being the ratio of 3:1 (vector: wild DNA) the more useful for obtained recombinant Iridovirus. Recombinant Iridovirus IIV-6, was obtained by co- transfection of vector DNA TOPO-295L-GFP-Cn10 and wild DNA Iridovirus IIV-6, using the technique of biolistic to co-infecting Galleria mellonella larvae. This recombinant Iridovirus expressed both proteins, the GFP and Cn10. Furthermore, using fluorescent microscopy, were detected a green fluorescent staining in few portions of fat tissue of G. mellonella larvae. The potential expression of GFP and Cn10 proteins, was corroborated by SDS- PAGE, restriction analysis and PCR. This is the first report of the production of a recombinant Invertebrate Iridovirus, expressing a reporter gene (GFP) and a virulence gene (Cn10) and represents a model system for the genetic improvement of Invertebrate Iridovirus. More studies are needed at the molecular level, such as the sequencing of the genome of recombinant Iridovirus IIV-6 and performing Western Blot tests to detect, for one hand, the insertion of both genes (GFP and Cn10) into the genome of IIV-6 Iridovirus, and on the other hand, to verified the correct expression of both proteins in the tissues of the infected insect larvae.. Poster / Viruses. Wednesday, 16:30. VI-10 Genomic sequencing and analysis of Sucra jujuba nucleopolyhedrovirus Xiaoping Liu, Feifei Yin, Zheng Zhu, Dianhai Hou, Jun Wang, Lei Zhang, Hualin Wang, Zhihong Hu, Fei Deng State Key Laboratory of Virology, Virus Resource and Bioinformatics Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, P.R. China Address for Correspondence: df@wh.iov.cn The complete nucleotide sequence of Sucra jujube nucleopolyhedrovirus (SujuNPV) was determined by 454 pyrosequencing. The SujuNPV genome was 135,952 bp in length with an A+T content of 61.34%. It contains 131 putative open reading frames (ORFs) covering 87.9% of the genome. Among these ORFs, 37 were conserved in all completely sequenced baculovirus genomes, 25 conserved in lepidopteran baculoviruses, 64 were found in other baculoviruses, and 5 were unique to SujuNPV genome. Seven homologous regions (hrs) were identified in the SujuNPV genome which can be classified into two groups. SujuNPV was identified to contain several duplicated or multiple copy genes, as it contains two copies of helicase, DNA binding protein gene (dbp) and cg30, 3 copies of inhibitor of apoptosis gene (iap), and 4 copies of baculovirus repeated ORF (bro). Phylogenetic analysis suggest that SujuNPV belongs to a subclade of group II alphabaculovirus, interestingly different from other baculoviruses, all the nine members of this subclade contain a second copy of dbp. Poster / Viruses. Wednesday, 16:30. VI-11 Functional analysis of exonuclease gene (012L) of Chilo iridescent virus Yeşim Aktürk Dizman 1,2 , Cemal Sandallı 2 , Zihni Demirbağ 2 and Remziye Nalçacıoğlu 1 1 Karadeniz Technical University, Faculty of Sciences, Department of Biology, Trabzon, Turkey 2 Recep Tayyip Erdoğan University, Faculty of Arts and Sciences, Department of Biology, Rize, Turkey Address for Correspondence: akturkyesim_53@hotmail.com Chilo iridescent virus (CIV) encodes an open reading frame (ORF 012L) homologous to exonuclease II of Schizo-saccharomyces pombe. In the current study, we focused on the characterization of exonuclease gene of CIV. The target gene was cloned into the pET28a vector, expressed in E. coli strain BL21 (DE3) Lys with an N-terminal His tag and purified to homogeneity by using Ni- NTA affinity chromatography. Biochemical characterization of the purified CIV-exonuclease protein (CIV-Exo) confirmed that this viral protein is a functional 5’-3’ exonuclease that digests 3’-biotin- labelled oligonucleotides and linear double-stranded DNA molecules from their 5’-termini in a highly processive manner. CIV-Exo has also a potent endonuclease activity in vitro. The CIV-Exo converted supercoiled plasmid DNA (replicative form I, RFI) into the open circular form (RFII) and then open circular form into linear form (RFIII). Both exonuclease and endonuclease activities of CIV-Exo are optimal at pH 8.0 in the presence of 10 mM MgCl2, 2 mM dithiothreitol and 100 μg BSA ml -1 . Poster / Viruses. Wednesday, 16:30. VI-12 Identification of a new multiple nucleopolyhedrovirus isolated from the Jasmine moth, Palpita unionalis (Hübner) (Lepidoptera: Pyralidae) in Egypt Regina G. Kleespies 1 , Yongjie Wang 2 , Said El Salamouny 3 , Mona Awad 3 , Essam Agamy 3 , Ramadan Salama 3 and Johannes A. Jehle 1,2 1 Institute for Biological Control, Julius Kühn Institute, Darmstadt, Germany; 2 Agricultural Service Station Palatinate, Neustadt/Weinstr., Germany; 3 Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt Address for Correspondence: ssalamo@clemson.edu A new multiple nucleopolyhedrovirus was isolated from diseased larvae of the jasmine moth, Palpita unionalis (Hübner) (Lepidoptera: Pyralidae) in Egypt. The virus caused typical symptoms of a baculovirus infection, and it was possible to propagate the causative agent in larvae of the homologous host. Light microscopy studies showed polyhedral occlusion bodies (OBs). Electron microscopy of ultrathin sections of polyhedral OBs showed multicapsid virions identifying the virus as a multiple embedded nucleopolyhedrovirus. Therefore, this virus was termed Palpita unionalis multiple nucleopolyhedrovirus (PaunNPV). The identity of the isolated virus was confirmed by sequencing of a 452 bp fragment of the polyhedrin (polh) gene that was amplified using degenerate primers. Blast search showed that it was closely related to polh genes in Dirphia peruvianus NPV, Pterolocera amplicornis NPV, and Nepytia phantasmaria NPV. A neighbour-joining phylogenetic tree was constructed based on the predicted amino acid sequences of the polh genes of the selected closely related NPVs. Phylogenetic distances suggested that PaunNPV should be considered to belong to a novel species within the genus Alphabculovirus. Preliminary bioassay data showed that the virus was active against either 2 nd or 4 th instars of jasmine moth. The calculated