Copyright © European Society of Anaesthesiology. Unauthorized reproduction of this article is prohibited. ORIGINAL ARTICLE Impact of nitrous oxide on the haemodynamic consequences of venous carbon dioxide embolism An experimental study Pierre A. Diemunsch, Eric Noll, Julien Pottecher, Michele Diana, Bernard Geny and Girish P. Joshi BACKGROUND Nitrous oxide (N 2 O) is still considered an important component of general anaesthesia. However, should gas embolisation occur as result of carbon dioxide (CO 2 ) pneumoperitoneum, N 2 O may compromise safety, as the consequences of a gas embolus consisting of a combi- nation of CO 2 and N 2 O may be more severe than CO 2 alone. OBJECTIVE This experimental study was designed to com- pare the cardiopulmonary consequences of gas embolisa- tion with a N 2 O/CO 2 mixture, or CO 2 alone. DESIGN Experimental study. SETTING Research Institute Against Digestive Cancer laboratory, Strasbourg, France. ANIMALS Seven Large-White pigs receiving standardised inhalation anaesthesia. INTERVENTIONS Each animal, acting as its own control, was studied in two successive experimental conditions – intravenous gas injections of 2 ml kg 1 of 100% CO 2 and 2 ml kg 1 of a gas mixture consisting of 10% N 2 O and 90% CO 2 . MAIN OUTCOMES MEASURES Haemodynamic and venti- latory consequences of embolisation with the gases . RESULTS We found that the haemodynamic (heart rate, mean arterial blood pressure, central venous pressure, mean pulmonary artery pressure, pulmonary artery occlusion pres- sure and transoesophageal echocardiography parameters) and ventilatory (arterial oxygen saturation, end-tidal CO 2 concentration and mixed venous oxygen saturation) con- sequences of embolisation with either 100% CO 2 or 10% N 2 O with 90% CO 2 were similar. CONCLUSION The findings of this study may alleviate concerns that the use of N 2 O, as a part of a balanced general anaesthesia technique, may have greater adverse consequences should embolisation of pneumoperitoneal gas containing N 2 O occur. Published online 28 November 2015 Introduction Nitrous oxide (N 2 O) is utilised as a part of a balanced anaesthesia technique because of its amnesic and analge- sic properties. 1 Although the role of N 2 O in modern anaesthesia remains controversial, 2,3 it could be beneficial in reducing awareness with recall, reducing persistent postoperative pain, favouring haemodynamic stability particularly in patients with high cardiovascular and neurological risks, and enabling rapid emergence from general anaesthesia. 1 Nevertheless, there is a concern that during laparoscopy, N 2 O anaesthesia may compromise safety should gas embolisation occur. 4–6 Previous studies have reported negative haemodynamic effects of N 2 O anaesthesia on venous gas embolism with 100% carbon dioxide (CO 2 ). 5,6 However, these studies did not assess the effects of gas embolism with a com- bination of CO 2 and N 2 O, which can occur during N 2 O anaesthesia, because N 2 O diffuses into the CO 2 pneu- moperitoneum. When embolisation of a mixture of CO 2 and N 2 O occurs from the abdominal cavity, N 2 O anaesthesia may prevent the elimination of N 2 O from the gas bubbles because of Eur J Anaesthesiol 2016; 33:356–360 From the Service d’Anesthesie Reanimation Chirurgicale, Hopitaux Universitaires, Strasbourg, France (PAD, JP, EN); IRCAD-EITS, Strasbourg, France (MD); Laboratoire de Physiologie EA 3072, Faculte ´ de me ´ decine, Strasbourg, France (BG); and Department of Anesthesiology, and Pain Management, University of Texas Southwestern Medical Center, Dallas, Texas, USA (GPJ) Correspondence to Professor Pierre A. Diemunsch, Service d’Anesthesie Reanimation Chirurgicale, Hopitaux Universitaires, 1 Avenue Molie ` re, Strasbourg, France, 67098 Tel: +33 38812076; fax: +33 388127074; e-mail: pierre.diemunsch@chru-strasbourg.fr 0265-0215 Copyright ß 2016 European Society of Anaesthesiology. All rights reserved. DOI:10.1097/EJA.0000000000000384