Proceeding of the 3
rd
International Conference on Bioscience and Biotechnology, Vol. 3, 2018, pp. 1-9
Copyright © 2018 TIIKM
ISSN 2513-2695 online
DOI: https://doi.org/10.17501/biotech.2018.3101
Corresponding Author Email: *raya_aa@mail.ru
STUDY OF SPRING WHEAT VARIETIES FOR
IDENTIFICATION OF FORMS RESISTANT TO
CADMIUM
R.A. Alybayeva*, M.F. Mulyukova, A.E. Bektiyar, V.I. Kruzhayeva, S.D. Atabayeva
and S. Sh. Asrandina
Kazakh National University named after al-Farabi, Almaty, Republic of Kazakhstan
Abstract: The purpose of our study was the identification of spring wheat varieties resistant to
cadmium, a priority pollutant in the East Kazakhstan region. The experiments were carried out in the
condition of model environmental pollution on 14-day sprouts of various spring wheat varieties, that
were grown on a nutrient mixture, containing 0.1 mM of CaSO4 and Cd ions at a concentration of 40
mg/l. Screening of spring wheat varieties under the conditions of the model experiment made it
possible to identify resistant and sensitive genotypes. In terms of growth and accumulation of
cadmium in the aboveground organs, the most resistant to cadmium spring wheat varieties are Samal
and Kaiyr, the least resistant are the varieties Lutescens and Zhenis. In terms of growth and
accumulation of cadmium in the roots, the most resistant varieties of spring wheat were Kazakhstan-
15 and Kazakhstan Early, the most unstable – the variety Lutescens. Chlorophyll a is more sensitive
to the action of cadmium ions than chlorophyll в. Cadmium-resistant varieties can be used in field
studies of soil contamination with cadmium to identify promising forms that combine metal
resistance with high yield and resistance to weather conditions.
Keywords: cadmium, spring wheat, growth parameters, cadmium content, chlorophyll content,
sensitive and resistant genotypes
Background. Objectives and goals
Pollution of the environment, in particular by chemicals, is one of the most powerful factors of destruction of
the biosphere components. At present, a large number of pollutants enter the biosphere. Among them, a
significant place is occupied by heavy metals. Heavy metals are the most toxic among chemical elements [1].
One of the ways to obtain clean commercial products on soils contaminated with heavy metals is to create, and
use in the production, technogenic resistant varieties of agricultural crops. The development of this method
raises the initial task of studying gene pool of the cultivated and wild plants and allocation of donors,
accumulating minimum amount of contaminants in the commercial part of the crop [2]. The evaluation of
selection material and the direction of researches on the principles of the use of attributes, that allow to
accumulate a minimum number of ecotoxicants, will make it possible to reduce terms of selection work [3,4]. In
connection with the task of studying the gene pool of cultivated plants in conditions of technogenic pollution,
the subject of the study was the screening of varieties of spring wheat for metal resistance in order to identify
the promising types for cultivation in the East Kazakhstan region, and selection donors that accumulate
minimum amount of pollutants.
Methods
The objects of research are different genotypes of spring wheat from the collection of the Kazakh Research
Institute of Agriculture (KIA): Kazakhstan Early, Erythrospermum, Kaiyr, Lutescens, Zhenis, Kazakhstan-25,
Samal, Samgau, Almaken, Kazakhstan-15.