~ 45 ~ Journal of Pharmacognosy and Phytochemistry 2022; 11(4): 45-50 E-ISSN: 2278-4136 P-ISSN: 2349-8234 www.phytojournal.com JPP 2022; 11(4): 45-50 Received: 05-05-2022 Accepted: 14-06-2022 Ankita Kulshreshtha Department of Chemistry, Institute for Excellence in Higher Education, Kaliyasot Dam, Kolar Road, Bhopal, Madhya Pradesh, India Jyoti Saxena Department of Chemistry, Institute for Excellence in Higher Education, Kaliyasot Dam, Kolar Road, Bhopal, Madhya Pradesh, India Corresponding Author: Ankita Kulshreshtha Department of Chemistry, Institute for Excellence in Higher Education, Kaliyasot Dam, Kolar Road, Bhopal, Madhya Pradesh, India Qualitative and quantitative estimation of phyto constituents in different solvent extracts of leaf of Tabernaemontana divaricata Ankita Kulshreshtha and Jyoti Saxena DOI: https://doi.org/10.22271/phyto.2022.v11.i4a.14446 Abstract Leaves of Tabernaemontana divaricata extracts in petroleum ether, ethanol chloroform, methanol and aqueous were analyzed for their phytochemical screening. Qualitative and quantitative phytochemical analysis was performed to identify the presence of various phytoconstituents with standard procedures. The presence of Alkaloids, Glycosides, Flavonoids, Diterpenes, Phenol, Proteins, Carbohydrate, Saponins and Tannins. For quantitative estimation of total phenols, total flavonoids, total alkaloid, total chlorophyll and total ascorbic acid were studied. The present study concluded that T. divaricata plant has the ability to cure a variety of diseases because it contents valuable phytochemicals. Keywords: Tabernaemontana divaricata, leaf, phytochemicals, phytochemical screening, qualitative analysis, quantitative analysis 1. Introduction The Tabernaemontana divaricata plant is an evergreen shrub growing to a maximum height of six feet and found in all parts of the India. Normally a Plant produces various metabolic products for their growth and development. Phytochemicals are naturally present in plants and they have biological significances. They play an important role in the plant growth or defending various pathogenic microbes [1] . The number of phytochemicals present in plants varies; some produces high amount were some produces less [2] . The components which are essential for the growth and survival for the producer plant are known as primary metabolites. Secondary metabolites are plant substances which are derived biosynthetically from primary metabolites. Flavanoids, alkaloids, terpenoids, phenols, tannins, saponins, steroids etc belong to this class. Previous studies have reported the phytochemistry and presence of associated chemicals like alkaloids, terpenoids, steroids, flavonoids, phenyl propanoids, phenolic acids and enzymes in the leaves, stems, and roots [3-4] . A variety of chemical compounds extracted from many parts of Tabernaemontana species reportedly contain alkaloids, which exhibit biological activities, such as antimicrobial, antioxidant [5-6] , anti-inflammatory,anticholinesterase, antineurodegenerative, anticancer, antidiabetic, antivenom, larvicidal, antihypertensive, wound healing, analgesic, astringent, antioxidantanxiolytic, anti diabetic and anticonvulsant [7-9] and many other activities [10-15] . In this study, qualitative and quantitative phytochemical analysis was performed to identify the presence of various phytoconstituents in different leaf extract of T. divericata with standard procedures. 2. Methodology 2.1. Plant material Leaves of T. divaricata were collected from local area of Bhopal in the month of July, 2020. Drying of fresh plant parts was carried out in sun but under the shade and then ground into coarse powder for extraction procedure. 2.2 Extraction Procedure 200 gm of shade dried leaves of T. divaricata were extraction with petroleum ether using maceration method. The extraction was continued till the defatting of the material had taken place [16-17] . Defatted plant leaf material were extracted in four solvents of different polarity viz water, methanol, ethyl acetate and chloroform by maceration method. The resultant content was filtered with whatman filter paper no.1 and kept for evaporation of solvent to get the dry