Pak. J. Biotechnol. Vol. 15 (1) 145-150 (2018) ISSN Print: 1812-183 www.pjbt.org ISSN Online: 2312-7791 EFFECT OF SUPERCYREN PESTICIDE IN THE HEART AND EYE TISSUES OF MICE Sabah A. Hameid, A. Rahman and Jehan M. Rajab Department of biology, College of Science, Al-Mustansiriyah University, Iraq E. mail: zainsamawal@yahoo.com Article received 7.1.2018, Revised 12.2.2018, Accepted 20.2.2018 ABSTRACT Organophosphorus insecticides constitute a large family of pesticides and they are used widely for controlling pests in the household, agricultural and urban environment, the present study aims to study effect of supercyren pesticide in the heart and eye tissues of mice . about Thirty albino male mice divided into three groups treated orally with supercyren pesticide in dose (ze, o,10, 20 mg /kg). After 30 days of the experiment the troponin protein was measured in blood samples of three groups for one month. For histological study samples of the heart , retina and cornea were collected and prepared with paraffin technique and stained with Hand E stain. The blood samples were negative for troponin protein in the control group and were positive at doses of (10mg and 20mg). The histological sections showed some changes in the heart and eyes with dose )10 and 20 mg/ kg (. The present study concluded harmful effects of various concentrations of supercyren in some body organs. Key words: pesticides, troponin , heart , retina, Organophosphorus INTRODUCTION Organophosphorus insecticides constitute a large family of pesticides and they are used widely for controlling pests in the household, agricultural and urban environment [Kacham et.al., 2006]. Chlorpyrifos is one of organic phosphorous compound used in different aspects throughout life, such such as in agricultural fields it is also used in house pest control throughout the world [ Saulsbury etal., 2009]. Chlorprifos has pharmackinetic mode similar to other organic phosphorous compound by inhibited acetylcholine esterase activity [Kaur and Sandhu,2008]. Chlorprifos effected in pregnant rats was inhibited materialfetal foetal brain activihours within 24 hr. [Zheet altal., 2000]. The most powerful effect of chlorpyrifos poisoning is dermal absorption especially in workers with means of plant protection on the other hand, certain pestiside have composed of two or three active ingredients in order to decreased poisoning effect of its [Zheng etal., 2000]. The modern use of insecticides is substantially improving the economic and social well being of the inhabitants of developing world by increased food production and by the effective control of public health vector-borne diseases, blood disorders, brain and nerve damage, cancer infertility (sterility) are often associated with low residual level of pesticides [Hatice and Kalendor , 2011]. In animals , interrupted the estrous cycle, decreased appetite or reproductive problems, decreased milk production can be correlated with continues low-level exposure of pesticides [Kalender et al., 2010]. The aim of this study was investigated the toxic effects of different pesticide concentrations in troponin level and histological changes in heart and eye (retina and cornea) in adult male mice. MATERIALS AND METHODS Thirty albino male mice weighting 140- 180 gram were housed in separated cages in the animal house at College of Science - Al-Mustansiriyah University under laboratory conditions ( Temperature 20-25 c ) were performed at January, 2017. The mice were divided into three groups each (10 mice) , group one (control ), group two orally administrated supercyren pesticide (10%) a dose (10 mg /kg/ BW) for one month , group three was administrated supercyren pesticide given daily at dose (20 mg/kg/BW).All mice were supplied with feed and water during the period of experenimt ( one month). using ( material active: 500 g /l chlorpyrifos and 50 g / l cypermethrin). Blood samples from each group were collected by direct cardiac puncturethen serum separated by centrifuging (3000 rpm) for (15 minutes) after this it transferred to freezer at( 20c) until analysis using enzyme immunoassay (ELIZA) . Heart and eyes were dissected out , cleaned with physiological saline solution (0.9 % NaCl ) and fixed with 10% buffered formalin about 7 days