Agro-knowledge Journal, vol. 19, no. 2, 2018, 89-97 89 Original scientific paper Оригиналан научни рад UDC 636.2:57.047(497.5) DOI: 10.7251/AGREN1802089J Determination of the Effect of Daily Production Level of Primiparous Holstein Cows on Response to Heat Stress Conditions (THI Threshold) in Eastern Croatia Ivana Jožef 1 , Maja Gregić 2 , Tina Bobić 2 , Božo Važić 3 , Vesna Gantner 2 1 University in Tuzla, Faculty of Technology, Federation of BiH, BiH 2 J.J. Strossmayer University in Osijek, Faculty of Agriculture, Croatia 3 University of Banja Luka, Faculty of Agriculture, Republic of Srpska, BiH Abstract The goal of this paper was to determine the effect of daily production level of primiparous Holstein cows kept on dairy cattle farms in Eastern Croatia on response to heat stress conditions (THI threshold). In order to do this, individual test-day records with data on ambient temperature and relative humidity in the barns were analysed. The data were collected in regular milk recording from 2006-2012. The THI threshold values for daily milk yield were determined by least square analyses of variance for each given THI value (from 68 to 72) using the PROC MIXED (SAS). Low-producing cows experienced significant drop at THI=68, while in high-producing primiparous Holsteins the first significant drop in daily milk yield was observed when THI=69. Also, the amount of daily production drop depended on the cow’s product ion level (0.155-0.256 kg/day in high-producing; 0.319-0.460 kg/day in low-producing). It could be concluded that the response of primiparous Holstein cows kept in Eastern Croatia to heat stress conditions depends on daily production level. Key words: primiparous Holsteins, heat stress conditions, temperature- humidity index, daily production level, Eastern Croatia brought to you by CORE View metadata, citation and similar papers at core.ac.uk