Journal of Aquatic Science and Marine Biology Volume 2, Issue 2, 2019, PP 33-39 ISSN 2638-5481 Journal of Aquatic Science and Marine Biology V2 ● I2 ● 2019 33 Factors Affecting Fish Blood Profile: C Effect of Other Environmental and Genetic Factors Abdelhamid M. Abdelhamid 1* , Mohamed M. A. H. Refaey 1 , Mahmoud F. Salem 2 and Mostafa A. M. El-Kattan 1 1 Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt 2 Aquaculture Research Unit, Sakha, Central Lab. of Aquaculture Research, Agricultural Research Center, Cairo, Egypt *Corresponding Author: Abdelhamid M. Abdelhamid , Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt, Email:abdmohabd@hotmail.com INTRODUCTION Penzlin (1977) cited that blood constituents of fish are variable than those of the terrestrial animals. Abdelhamid (1991) argumenta for the variation between fish and terrestrial animals to the huge number of fish species (ca. 25.000) and strains (40.000) in comparison with the mammalian species (ca. 4.500). Additionally, Abdelhamid (2009) cited that fish are variable in their morphology, biology, anatomy, physiology, reproduction, respiration, body composition, and blood profile. Blood parameters of fish are useful tools that aid in the immnuopotentiators studies (Ellis, 1981). Press and Evensen (1999) expected species variation in the morphology of the immune system within the teleost fishes. However, data available about the on to genic development of the innate immunological system in fish is limited (Magnadóttir, 2006). Recently, some factors were studied as affecting fish blood profile (Abdelhamidet al., 2019a & b). The present study was designed to evaluate the effect of some other factors affecting some blood parameters. These factors included fish species and size, water salinity, and rearing conditions; since, it was given in Verse 12 of SuraFaatir of Holly Quran concerning the variations in water and fish composition: "And not alike are the two bodies of water. One is fresh and sweet, palatable for drinking, and one is salty and bitter. And from each you eat tender meat and extract ornaments which you wear, and you see the ships plowing through [them] that you might seek of His bounty; and perhaps you will be grateful ". So, this was also included in the objectives of the present study. MATERIALS AND METHODS Random fish samples were taken for blood collected from the caudal peduncle by special syringe. Five-ml-syringes were used for blood withdrawal into two types of test tubes, the 1 st with ethylene diamine tetra acetic acid (EDTA) for the haematological parameters and the 2 nd without EDTA for the biochemical parameters. Blood plasma was obtained by centrifugation at 3500 rpm for 15 min. Dragoon pipets were used for withdrawing the samples from the test tubes as shown from the following pictures. Blood plasma samples were used for determination of creatinine (Tietz, 1986), triglycerides (Mc Gowan et al., 1983), total proteins (Tietz, 1990) and albumin (Wotton and Free man, 1982) concentrations as well as the activity of aspartate amino transferase (AST) and alanine amino transferase (ALT) using commercial test kits (Humalyzer 3000 manufactured by Human, Germany) in a private lab. In Kafr El-Sheikh ABSTRACT Many studies in the fields of fish nutrition, reproduction, and biology including blood tests revealing factors influence blood composition. In the present part of the serial studies, more lights are given on effects of fish species, sex and size as well as different water bodies' locations (lake, farm, Nile) and water salinity and temperature on fish blood changes. Keywords: Haematology, blood biochemistry, fish species, size, sex, fresh water, salt water, brackish water, water temperature.