Research Article Probiotic Potential and Safety Characterization of Enterococcus hirae G24 isolated from Indigenous Raw Goat Milk Kamni Rajput * , Ramesh Chandra Dubey Department of Botany and Microbiology, Gurukula Kangri Vishwavidyalaya, Haridwar-249404, Uttarakhand, India Introduction Fresh milk and nutritious milk food are introducing a complex probiotic microbiota. Fresh milk is commonly recognized that the lactic acid bacteria (LAB), including Enterococcus genus, are the prevailing population, such as, cow, goat, sheep, and buffalo milk. [1] Goats were among the main farm animals to be tamed for about numerous years. [2] Goat milk contains lipids, protein, carbohydrates, vitamins, and minerals. Its composition is a higher correlation with other bovine milk except for the presence of a low amount of lactose. Milk fat globules are littler and perhaps, one of the explanations behind the simple processing of this milk. High source of protein, fat, phosphate, and calcium are found in goat milk products. Its composition depends on many factors, such as, diet, assortment, environment, and conditions. Goat milk Article history: Received: 21 January, 2020 Revised: 12 April, 2020 Accepted: 27 April, 2020 Published: 30 May, 2020 Keywords: Enterococcus hirae, Goat milk, Lactic acid bacteria, Probiotic, Probiotic attributes safety parameters. DOI: 10.25004/IJPSDR.2020.120303 In this paper, an investigation on lactic acid bacterial isolates from ethnic goat raw milk samples were examined for their probiotic potential and safety parameters. For this purpose, isolated bacterial cultures were screened based on certain parameters, viz., sugar fermentation, tolerance to temperature, salt, low pH, bile salts, and phenol resistance. After that, these bacterial cultures were more estimated in vitro for auto-aggregation, cell surface hydrophobicity, response to simulated stomach duodenum channel, antibiotic resistance, and antimicrobial activity. Besides, probiotic traits show the absence of gelatinase, and hemolytic activity supports its safety. The isolate G24 showed good viability at different pH, bile concentration, phenol resistance, and response to simulated stomach duodenum passage, but it did not show gelatinase and hemolytic activities. Isolate G24 was susceptible to amikacin, carbenicillin, kanamycin, ciprofloxacin, co-trimazine, nitrofurantoin, streptomycin, and tetracycline. Isolate G24 also exhibited antimicrobial action against five common pathogenic bacteria, such as, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogens, and Salmonella typhimurium. It displayed the maximum auto-aggregation, cell surface hydrophobicity to different hydrocarbons. Following molecular characterization, the isolate G24 was identified as Enterococcus hirae with 16S rRNA gene sequencing and phylogeny. E. hirae G24 bears the excellent properties of probiotics. Contents lists available at UGC-CARE International Journal of Pharmaceutical Sciences and Drug Research [ISSN: 0975-248X; CODEN (USA): IJPSPP] journal home page : http://ijpsdr.com/index.php/ijpsdr International Journal of Pharmaceutical Sciences and Drug Research 2020; 12(3): 218-226 *Corresponding Author: Kamni Rajput Address: Department of Botany and Microbiology, Gurukula Kangri Vishwavidyalaya, Haridwar-249404, Uttarakhand, India Email : kamnirajput20@gmail.com Tel.: +91-8954541157 Relevant conflicts of interest/financial disclosures: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Copyright © 2020 Kamni Rajput et al. This is an open access article distributed under the terms of the Creative Commons Attribution- NonCommercial- ShareAlike 4.0 International License which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. ABSTRACT ARTICLE INFO has better absorbability, alkalinity, buffering capacity, and certain remedial qualities in medication and human nutrition than that of other bovine milk. [3] Enterococci are gram-positive, catalase-negative, no spore-forming cocci, and occur in vegetables, plant materials, and various dairy products. Enterococci relate to lactic acid bacteria and significant in food due to fermentation as probiotics in human and animals. Enterococci directly contribute to the distinctive flavor and taste of traditional dairy products. They also protect many fermented foods, such as, sausages, cheese, and dairy products. [4] Several species of Enterococcus have been commonly used as probiotics in humans and animals for the cure of gastroenteritis. [5] Probiotics are also used in microbial food supplements, which apply different human health medical advantages,