_____________________________________________________________________________________________________ *Corresponding author: E-mail: dr_rashavet22@yahoo.com; Annual Research & Review in Biology 29(3): 1-11, 2018; Article no.ARRB.44651 ISSN: 2347-565X, NLM ID: 101632869 Advanced Studies on Virulence Genes of Salmonella and Shigella species Isolated from Milk and Dairy Products Gamal A. M. Younis 1 , Rasha M. Elkenany 1* and Wesam S. Abd-Elmoati 1 1 Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. Authors’ contributions This work was carried out in collaboration between all authors. Author GAMY designed the study, performed the statistical analysis and wrote the protocol. Authors RME and WSA wrote the first draft of the manuscript and managed the analyses of the study and the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/ARRB/2018/44651 Editor(s): (1) Dr. Manikant Tripathi, Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad, India. (2) Dr. George Perry, Dean and Professor of Biology, University of Texas at San Antonio, USA. Reviewers: (1) Vincent Habimana, University of Nairobi, Kenya. (2) R. Prabha, Karnataka Veterinary, Animal and Fisheries Sciences University, India. Complete Peer review History: http://www.sciencedomain.org/review-history/27108 Received 06 August 2018 Accepted 12 October 2018 Published 08 November 2018 ABSTRACT Salmonella and Shigella species are the main health problem in various portions of the world. This study gave rise to detect and enumerate Salmonella and Shigella species with detection of virulence genes by PCR in randomly collected raw milk and dairy products (ice cream, cheese, yoghurt, rice with milk and cream) from different vendors of village and dairy farms in Mansoura Governorate, Egypt during October 2016. A total of 24 (9.6 %) isolates from 250 samples (raw milk and dairy products) were recognised as Salmonella 6.4 % (16/250) and Shigella 3.2 % (8/250) species with their high prevalence in raw milk. Amongst serotypes of Salmonella species: S. Typhimurium 37.5 % (6/16), S. Enteritidis, S. Tsevie 18.75 % (3/16 each) and other serovars 25 % (4/16). Additionally, the identified Shigella species (8/250) were S. dysenteriae 50 % (4/8), S. flexneri 25 % (2/8) and S. sonnei 25 % (2/8). The average of total viable count of samples positive for Salmonella and Shigella in raw milk and dairy products was 4.47±0.97 log 10 CFU/ml or gm and 4.27±1.01 log 10 CFU/ml or Original Research Article