International Journal of Health and Pharmaceutical https://ijhp.net 215 Test Of Antioxidant And Antibacterial Activity Of Ethanol Extract Of Andaliman Fruit (Zanthoxylum Acanthopodium Dc.) With Dpph (1.1-Diphenyl-2- Picrylhydrazil) Trapping Method And Minimum Inhibitory Concentration Ira Syaputri 1 , Ermi Girsang 2* , Linda Chiuman 3 1,2,3 Program Study Master Of Biomedic Science Faculty Of Medicine Universitas Prima Indonesia Medan, North Sumatera, Indonesia * Corresponding author: Email: ermigirsang@unprimdn.ac.id Abstract. Utilization of bioavailability according to World Health Organization (WHO) records is very large, it is estimated that almost 80% of mankind, especially in developing countries, still use plants as medicinal ingredients to maintain their health (Nugraheni, 2007). Andaliman (Zanthoxylum acanthopodium DC.) which belongs to the Rutaceae tribe which is one of the plants known to the Batak community, is classified as a wild plant and is a typical plant of Province North Sumatra. The method used in this research is experimental research. The research included collecting and processing andaliman fruit, making simplicia, making ethanol extracts, and examining antioxidant activity tests using the DPPH free radical scavenging method (1,1-diphenyl-2-picrylhydrazil) and antibacterial activity using the minimum inhibitory concentration method. The results of the antibacterial activity test of the ethanolic extract of andaliman fruit showed that the extract had antibacterial activity in the moderate category at a concentration of 300 mg/ml with an inhibition zone of 10.17 mm for Staphylococcus aureus bacteria and a moderate category for Staphylococcus epidermidis bacteria with an inhibition zone of 10.80 mm. The ethanol extract of andaliman fruit can inhibit Staphylococcus aureus and Staphylococcus epidermidis, effective at a concentration of 300 mg/ml with inhibitory diameters of 10.17 mm and 10.80 mm, respectively. Keywords: Andaliman fruit, antioxidants, DPPH, Staphylococcus aureus, and Staphylococcus epidermidis. I. INTRODUCTION. Utilization of bioavailability according to World Health Organization (WHO) records is very large, it is estimated that almost 80% of mankind, especially in developing countries, still use plants as medicinal ingredients to maintain their health (Nugraheni, 2007).In general, plants produce secondary metabolites that can be used to treat various types of diseases. The secondary metabolite compounds are alkaloids, flavonoids, saponins, tannins, steroids, and triterpenoids (Harborne, 1987). The ability of a plant is supported by the secondary metabolites contained in it. Climatic factors which include air temperature, sunlight, air humidity, and wind and soil conditions greatly affect the plant growth process to the variation of secondary metabolites contained.The effect of the material is closely related to the chemical compounds contained in the material. Andaliman plants contain terpenoid compounds (Wijaya, 1999), phenols, and steroids (Suryanto, et al., 2008). Phenol compounds are bioactive components that are toxic to predatory animals (Robinson, 1995). Several studies have shown that andaliman fruit extract has anti-inflammatory activity, immunostimulant effects, and antioxidant compounds in spices can also trigger the immune system, especially anticancer activity (Siregar, 2003).Based on several studies on medicinal plants, it was reported that many medicinal plants contain large amounts of antioxidants. The antioxidant effect is mainly due to the presence of phenolic compounds such as flavonoids and phenolic acids. Usually, the compounds that have antioxidant activity are phenolic compounds that have hydroxyl groups distributed in the ortho and para positions concerning the –OH and –OR groups (Praptiwi, et al., 2006).