International Journal for Multidisciplinary Research (IJFMR) E-ISSN: 2582–2160, Volume: 4, Issue: 4, July-August 2022 Evaluation of Proprietary MDZenPro Formulation by Zenherb Labs in Mediating Protein Digestion under INFOGEST In-vitro Simulated Gastrointestinal Conditions Mihir Gadani, Ratna Upadhyay, Supriya Raut, Sneha Badak Department of Phytochemistry, Zenherb Lab Pvt. Ltd., Mumbai, India Highlights of the Work (1) The study has evaluated and reported the efficiency of a proprietary formulation named ‘MDZenPro’ in digesting proteins using an in-vitro simulated digestion. model. (2) MDZenPro was found to enhance the degree of hydrolysis of the proteins. This was supported by the results of SDS-PAGE. (3) The results of the study confirm the application of MDZenPro in aiding digestion. Abstract Protein breakdown by endogenous enzymes in the gastrointestinal (GI) tract results in generation of peptides and amino acids that act as building blocks in essential biological functions. Exogenous proteases possess immense potential as digestive enzyme supplements that can assist protein digestion in the GI system. Plant proteases, in addition to their promising activity, are considered to be safe in nature. The present study evaluated the potential application of a plant protease based proprietary formulation - MDZenPro in digesting raw whey protein, whey protein isolate and plant protein under INFOGEST simulated GI conditions. The gastric and GI digested protein products were analyzed for determining the degree of hydrolysis. The protein profiles were evaluated using SDS-PAGE. The results of the degree of hydrolysis study revealed that MDZenPro facilitated gastric and GI digestion of proteins. This increase in degree of hydrolysis was noted to be higher than that observed in proteins that were not treated with MDZenPro. The SDS-PAGE profile further supported these findings wherein, the MDZenPro treated protein samples displayed low molecular weight fragmented peptides in contrast to the profile of undigested proteins. The present study thus highlights the promising application of ‘MDZenPro’ as an effective supplement for protein digestion. Keywords: Protease, Digestion, MDZenPro, Simulated Gastrointestinal Digestion Introduction In recent times, digestive enzyme supplements involved in augmentation of gastrointestinal (GI) digestion have gained immense popularity. Such digestive supplements mainly consist of exogenous IJFMR2204012 Website : www.ijfmr.com Email : editor@ijfmr.com 129