Introduction Use of bio-agent for control of several plant diseases is an alternative approach in comparison of chemical fungicides because these are safe, effective and eco-friendly in comparison of chemical (Ramamoorthy et al., 2002). The most commonly used bioagents are , Gliocladium virens Trichoderma harzianum, T. veridae, Agrobacterium radiobacter , Pseudomonas fluorescens, Baccillus subtilis, soil-borne fungi, bacteria and Actinomycetes as antagonist against several pathogenic microbes (Ramamoorthy et al., 2002; Agrios, 2005; Gravel et al., 2005; Intana et al., 2008; Montealegre et al., 2010; Khare et al., 2010; Christy Jeyaseelan et al., 2012). The antagonistic reactions include antibiosis, competition and hyper parasitism of (Cook and Baker, 1983; Leelavathi et al., 2014). It is reported that various species of secret Trichoderma approx 40 different types of antibiotics, chemical compounds and many secondary metabolites against microorganisms specially gram positive and negative bacteria. These chemicals or metabolites promote plant growth and yield of crop (Tapwal et al., 2011; Mukherjee et al., 2013; Ruano-Rosa et al., 2014). Bacterial leaf spot (BLS) disease of sweet pepper (Capsicum annuum Xanthomonas axonopodis ) caused by pv. vesicatora is recorded from several countries of eastern and southern Africa, USA, Ethiopia, Kenya, Malawi, Mozambique and South Africa (Black et al., 2001). XAV suspected from symptoms on fruit was confirmed by isolation on semi-selective media including Tween B (McGuire et al., 1986; Jones et al., 2000). In India, bacterial leaf spot disease was first reported from Pune, Maharastra in 1948 by Patel in chilli. In Rajasthan, the disease caused et al. 7.5 to 16.6 per cent loss in the yield of fruits 22-34°C and high humidity for maximum infection (Shekhawat and Chakravarti, 1976; Shekhawat and Chakravarti, 1977). It losses in marketable fruits may be more than 50% (Pohronezny et al., 1992; Anonymous, 2012). The pathogen was found seed-borne (10-15%) and occurs on infected plant debris and weeds (Jones et al., 1986). The incidence was less than 5% persisted from one season to next in crop debris or on weed hosts (Ravinkar et al., 2001). Xanthomonas axonopodis vesicatora pv. (Doidge) Dye (syn: pv ) (XAV) a Xanthomonas campestris vesicatoria gram-negative, rod-shaped bacterium attack on plant (Anonymous, 1996; Thieme et al., 2005; Anonymous, 2016). The bacterial colonies were circular, raised, yellow, mucoid colonies on Tween-80 agar medium. The isolates were gram's negative, KOH solubility test positive, levan negative, lipase activity positive, oxidase negative, starch hydrolyzing, gelatin hydrolyzing, arginine variable, did not Bio-efficacy of fungal and bacterial antagonists against pv. Xanthomonas axonopodis vesicatoria Capsicum (Doidge) Dye in chilli ( spp.) grown in Rajasthan Dilip Kumar Sharma Vardhaman Mahaveer Open Universiy, Kota, Rajasthan, India https://doi.org/10.31024/ajpp.2018.4.2.18 * Corresponding Author: Address for Dr. Dilip Kumar Sharma Vardhaman Mahaveer Open Universiy, Kota, Rajasthan, India Email: drdilipsharma12@gmail.com Abstract Background: The fungal and bacterial antagonist were used to study their antimicrobial efficacy against Xanthomonas axonopodis vesicatoria pv. (Doidge) Dye in chilli causing bacterial leaf spot. The present study indicates Objective: the effective control of the pathogen and reduced the disease incidence significantly in non-hazardous manner by using fungal and bacterial antagonists. For the control of pathogen 5 fungal and 2 bacterial Materials and methods: antagonists namely , Gliocladium virens Trichoderma viride, T. harzinum, Stachybhotrys atra, Penicillium chrysogenum Bacillus subtilis, Pseudomonas fluorescence and respectively used by filter paper disc assay, seeded agar method and standard blotter methods. Out of used fungal antagonists found most Results: Trichoderma viride effective which improve seed germination and control of pathogen followed by as compared Penicillium chrysogenum to check in all the method. In filter paper method, bacterial antagonist found effective against the Bacillus subtilis pathogen followed by in control of various isolates of XAV. Pseudomonas fluorescence Keywords: Antibacterial, chilli, fungal antagonists, bacterial antagonists, pv. Xanthomonas axonopodis vesicatoria Received: 16 February 2018 Revised: 16 March 2018 Accepted: 20 March 2018 Research Article www.ajpp.in Asian Journal of Pharmacy and Pharmacology 2018; 4(2): 207-213 207