mode for vascular lesions compared with current NBI system. Methods: From September 2012 to May 2009, 14 cases (VE: vascular ectasia, 8 cases, GAVE: gastric antral vascular ectasia, 2 cases, RC: radiation colitis, 4 cases) were enrolled in this study. These cases were taken 28 images (close view: 14 image, distant view: 14 images) in the same situation using normal mode, NBI mode and monochrome mode (MONO mode), respectively. Normal mode observation was defined as 5 points. A total of 84 images (28 images by three modes) were evaluated on a scale of one to ten according to “a: Recognition of the lesion”, “b: Observation of the vessel” and “c: Observation of the background mucosa” by 15 trainee doctors who had few experience of endoscopy. Results: The scores of NBI mode (a: 5.37, b: 5.50, c: 5.06) and MONO mode (a: 5.33, b: 5.44, c: 5.02) were resulted almost the same evaluation, and they were better than normal mode. In the close view, the scores of NBI mode were better results according to the recognition of the lesion (a: 5.72 vs. 5.43, p 0.05) and the observation of the background mucosa(c: 5.51 vs. 5.17, p 0.05) compared with MONO mode. However, although there was no significant difference in the distant view, MONO mode (a: 5.23, b: 5.17, c: 4.86) was better result in all factors compared with NBI mode (a: 5.03, b: 5.01, c: 4.62). The brightness of the field of view obtained using MONO mode even in the distant view was considered as a reason of the results. Moreover, MONO mode was effective for observation of the blood vessel image remaining after APC ablation for RC and GAVE as evaluation of the endoscopic treatment. Conclusion: The monochrome mode is convenient and useful in the endoscope function for the observation of the vascular lesions. Mo1681 An Evaluation of Motility, Metabolic, Satiety, and Weight Changes Utilizing Endoscopically Placed Suture Anchors for the Treatment of Primary Obesity-Early Results RomáN Turró* 1 , Jorge C. Espinos 1 , Fermin Mearin 1 , Alfredo Mata 1 , Monica Isart 3 , Silvia Delgado-Aros 2 , Gloria Moragas 4 , JesúS Turró 1 1 Endoscopia Digestiva, Centro Medico Teknon, Barcelona, Spain; 2 Parc de Salut, Hospital del Mar, Barcelona, Spain; 3 Analisis Clinicos, Laboratorios Echevarne, Barcelona, Spain; 4 CETIR, Barcelona, Spain Background: Obesity is a complex, metabolic disease. Bariatric surgery offers the most significant and durable weight loss, improving quality of life and obesity related co-morbids. However, the associated risks of surgery precipitate the need for less invasive and safer alternatives. The Incisionless Operating Platform™ (USGI Medical, San Clemente, CA, USA) utilizes the g-Cath EZ ™ suture anchor, which has demonstrated the ability to durably plicate gastric tissue endoscopically at 2+ years. Prior work at our institution revealed mean weight loss of 22.5Kg at 1 year (N=13).This study evaluates the effect of suture anchor placement in the gastric fundus and at the antral inlet on gastric capacity, function, and body weight. Methods: A prospective, single site study was initiated with Ethics approval. Enrolled patients were between of the ages of 21- 60 with a BMI of  30 and  40 and no history of previous bariatric procedure. Serial nuclear gastric images over 3 hours (solid meal) were done pre-procedure and at 2/6 months. Serial peptide levels were recorded over 2 hours both pre- procedure and at 2 months in response to a standard solid meal. A standardized liquid satiety test (Ensure) was done before and at 2/6 months. Diet was advanced post procedure to full solids over 4-6 weeks to allow time for suture plication healing. Weights were recorded at 1, 2, 3, 4, and 6 months. Patients will be followed through 2 years. Results: 18 patients were enrolled (78%F/22%M). Mean age =39.1. At baseline: mean weight was 101.710.7Kg; mean body mass index (BMI) = 36.32.3. A mean of 9.7 plications were placed in the fundus, 3.9 at the distal gastric body. Mean operative time, 51 minutes (39.0-70.0). , , or significant post-operative to dateAll 6 month mean weight loss = 16.9 7.5 Kg (N=18); %Excess Weight loss (BMI25)/%Total Body Weight Loss was 55.1%/ 16.7%. Total gastric emptying rate slowed at all time points in 72% (13/18) at 2 months compared to baseline. Mean total gastric emptying half time (T50) was increased at 2 months (p=.119). Mean fundal T50 showed significant slowing at 2 months (p=.042), but was less significant at 6 months. Metabolic changes included a decrease in mean fasting/post-prandial sugars for all (N=18). Significant decreases in mean AUC at 1 hr. were observed with leptin and insulin (p.001), and 1in gastric inhibitory peptide (p=.004). All patients with baseline and 6 month data demonstrated reduced capacity and earlier satiety during testing. Conclusions: At early follow-up, this procedure appears to provide a safe and effective short term weight loss solution, and may decrease gastric capacity and produce earlier fullness through either a mechanical and/or physiologic mechanism. Longer-term follow-up and further study are required. Results Satiety testing N16 Mean Test Duration (Minutes) Mean Maximum Volume Tolerated (ml) Mean Maximum Calories Tolerated (Kcal) Pre-Procedure 39.7 567.1 898 6 Months Post- Procedure 24.4 p=. 001 351.5 p=. 001 591.3 p=. 001 Mo1682 High Resolution In Vivo Anatomical and Molecular Optical Imaging of Colon Carcinogenesis in a Mouse Model Sarah J. Leung*, Jennifer K. Barton Biomedical Engineering, University of Arizona, Tucson, AZ Using targeted contrast agents in combination with optical coherence tomography (OCT) and laser induced fluorescence (LIF) imaging modalities, we are able to track the in vivo expression of molecular markers during early stages of colon carcinogenesis. This is accomplished using a multimodal endoscope equipped with OCT and LIF optics and lavage-administered antibodies tagged with fluorescent Cy5.5 in an azoxymethane (AOM)-treated mouse model of colon cancer. The endoscopic imaging system enables non-destructive, high resolution and high sensitivity imaging. OCT provides information on the gross structural tissue changes. LIF, in combination with synthesized fluorescent contrast agents optimized for low signal background with tissue and the multimodal endoscope, provides sensitivity to shifts in the expression of molecular markers pertinent in cancer development. This combined system enables time-serial imaging of an individual animal’s disease development and regression.We monitored in vivo changes in expression of epithelial growth factor receptor (EGFR), transferrin receptor (TfR), transforming growth factor beta (TGF), and chemokine (C-X-C motif) receptor 2 (CXCR2) in the distal colon of AOM-treated mice. In vivo OCT and LIF imaging was validated against frozen histological sections of colon tissue collected after mouse sacrifice. The monochrome mode image for vascular lesions. NBI mode image for vascular lesions. Abstracts AB470 GASTROINTESTINAL ENDOSCOPY Volume 77, No. 5S : 2013 www.giejournal.org