ORIGINAL ARTICLE Prooxidant–antioxidant balance in patients with traumatic brain injury Mohamadreza Ehsaei • Mehdi Khajavi • Mohammad Hassan Arjmand • Mohammad Ali Abuee • Majid Ghayour-Mobarhan • Daryoush Hamidi Alamdari Received: 12 February 2014 / Accepted: 8 April 2014 Ó Belgian Neurological Society 2014 Abstract Brain trauma is an important cause of mortality and disability among young people worldwide. One of the mechanisms of post-traumatic secondary brain damage is related to free radical release and oxidative stress (OS). OS is the consequence of an imbalance between pro-oxidants and antioxidants in favor of pro-oxidants. This imbalance may lead to macromolecule damage including lipid peroxidation, protein crosslinking, DNA damage and changes in growth and function of cells in brain. Free radical release and subsequent lipid peroxidation are early events following neural tissues injury and are associated with hypo-perfusion, edema, and disruption of axonal guidance. In this study, we determined the prooxidant–antioxidant balance (PAB) in patients with brain injury, and its correlation with number of demographic and clinical parameters. Sera from 98 patients with traumatic brain and 100 healthy subjects were collected. The serum PAB was measured. Age, sex, GCS (Glasgow coma scale), mech- anism of injury, brain lesions found on CT scan and lesions in other parts of the body, caused by trauma, were determined. A significantly higher PAB value was observed in the patient group (138.97 ± 15.9 HK unit) compared to the controls (60.82 ± 12.6 HK) (P = 0.001). In the patient group, there was no significant correlation of PAB with GCS, brain lesion characteristic, mechanism of injury, other accompanying traumatic injury, age and gender. When patients were classi- fied into three groups according to GCS: group 1 (GCS [ 13, n = 28, PAB serum value = 138.51 ± 62.66 HK), group 2 (GCS between 8 and 12, n = 29, PAB serum value = 162.7 ± 50.6 HK) and group 3 (GCS\ 8, n = 41, PAB serum value = 155.56 ± 58.21 HK); there was no significant dif- ference between groups. The serum PAB values were higher in patients with traumatic brain injury, although this was not associated with the extent of injury. Keywords Prooxidant-antioxidant balance Á Trumatic brain injury Á Oxidative stress Abbreviation TOS Total oxidant status FOX Ferrous ion oxidation xylenol orange GCS Glasgow coma scale PAB Prooxidant–antioxidant balance TBI Traumatic brain injury ROS Reactive oxygen species Introduction There is a high prevalence of brain trauma in young people globally and hence the mechanisms underlying central M. Ehsaei Department of Neurosurgery, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran M. Khajavi Á M. A. Abuee Resident of Neurosurgery, Department of Neurosurgery, Emdad Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran M. H. Arjmand Master of Biochemistry, Department of Surgery, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran M. Ghayour-Mobarhan Biochemistry of Nutrition Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran D. Hamidi Alamdari (&) Biochemistry and Nutrition Research Center, Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran e-mail: Hamidiad@mums.ac.ir 123 Acta Neurol Belg DOI 10.1007/s13760-014-0300-4