International Journal for Multidisciplinary Research (IJFMR) E-ISSN: 2582-2160 ● Website: www.ijfmr.com ● Email: editor@ijfmr.com In Vitro Anthelmintic Properties of Euphorbia Milii and Euphorbia Microphylla Linn Extracts Dr. S. R. Kane 1 , Dr. S. V. Jawarkar 2 , Dr. A.M. Kandalkar 3 , Dr. S. K. Mohite 4 1 Research Scholar, Department of Pharmaceutical chemistry, Rajarambapu College of Pharmacy, Kasegaon. Shivaji University Kolhapur, India. 2 S. D. Patil Institute of Pharmacy, Islampur Sangli, India 3 L.S.R.G. Institute of Pharmacy, Akola, India 4 Principal, Rajaram Bapu COP, Kasegaon Abstract Methanol extracts from the leaves and stems of Euphorbia microphylla Linn and Euphorbia milii were investigated for their activity against Fasciola gigantica, Taenia solium and Pheritima pasthuma, respectively. Five concentrations (10–100mg/ml) of each extract were studied in the bioassay, which involved determination of time of paralysis and time of death of the worms. All the extracts exhibited con-siderable anthelmintic activities, and the order of sensitivity of the extracts to the worms was P. pasthuma > F. gigantica > T. solium. The most active of the extracts were E milii and E microphylla Linn stem methanol extracts. Piperazine citrate (10mg/ml) and distilled water were included in the assay as standard reference drug and control, respectively. Keywords: Euphorbia microphylla Linn, Euphorbia milii, anthelmintic, worms. Introduction Euphorbia milii and Euphorbia microphylla Linn are two plants belonging to the Euphorbiaceae. Plants from this family have been used in African traditional ethnomedicine for several years and several genera plants have been docu-mented for the treatment of various ailments. Plants of the Capparidaceae have been used for the treatment of syphilis, dressing of wounds, chronic ulcers, and treatment of snake bites (Dalziel, 1937; Kerharo & Adams, 1974). Certain plants in the plant family are also noted for the treatment of gon-orrhoea (Pernet, 1972), convulsion in children, aphrodisiacs (Ainsle, 1937), and, mostly, as anthelmintics (Dalziel, 1937; Walker, 1953; Irvine, 1961; Kerharo, 1968; Burkhill, 1985). The plant family is well known for the presence of glucosi-nolates or the so called mustard oil glucosides (Kjaer & Thompson, 1963; Ahmed et al., 1972). Alkaloids of the stachydrine-type are also well represented in this plant family (Delaveau et al., 1973). To a lesser extent, flavonoids and sterols have been indicated in a few of these plants (Bombardelli et al., 1973). Specifically, E milii stem bark has been used for the treatment of earache, bark decoction is drunk for chest pains, kidney pains and for washing small pox wounds. The fruit is anthelmintic. Seeds of E