Pak. J. Phytopathol., Vol. 34 (02) 2022. 173-185 DOI: 10.33866/phytopathol.034.02.0789 173 Official publication of Pakistan Phytopathological Society Pakistan Journal of Phytopathology ISSN: 1019-763X (Print), 2305-0284 (Online) http://www.pakps.com THE EFFICACY OF COST-EFFECTIVE BIONEMATICIDE AGAINST POTATO CYST NEMATODE GLOBODERA ROSTOCHIENSIS a Iis N. Asyiah*, a Dhania H. Tristaningtyas, a Jekti Prihatin, b Sugeng Winarso, c Lenny Widjayanthi, d Dwi Nugroho, e Kurniawan Firmansyah, f Ankardiansyah P. Pradana a Biology Education Study Program, Faculty of Teacher Training and Education, Universitas Jember. Jl. Kalimantan No. 37, Jember 68121, East Java, Indonesia. b Soil Science Study Program, Faculty of Agriculture, Universitas Jember. Jl. Kalimantan No. 37, Jember 68121, East Java, Indonesia. c Agricultural Extension Study Program, Faculty of Agriculture, Universitas Jember. Jl. Kalimantan No. 37, Jember 68121, East Java, Indonesia. d The Indonesian Coffee and Cocoa Research Institute, Jl. PB. Sudirman 90, Jember 68118, East Java, Indonesia. e Tiga Kreasi Bersama Limited Partnership (Commanditaire Vennootschap), Jl Sukowono No 144, Bondowoso 68263, East Java, Indonesia. f Plant Protection Study Program, Faculty of Agriculture, Universitas Jember. Jl. Kalimantan No. 37, Jember 68121, East Java, Indonesia. A B S T R A C T Potato cyst nematode (Globodera rostochiensis) infection causes yield loss of up to 80%. Various attempts have been made to suppress their infection on potato. However, G. rostochiensis infection remains a problem that has not been fully resolved. One of the potential techniques to control their population is the use of biological control agents. In previous studies, we have succeeded to isolate 3 rhizobacteria ( Bacillus sp.) and 1 endophytic bacterium (Pseudomonas dimunita) from the rhizosphere and root of the coffee plant. In this study, the bionematicide was formulated using four bacterial isolates and inexpensive materials. Bionematicides were tested for their effectiveness on land infected with G. rostochiensis (227 per 100 mL) of soil. We compared the effectiveness of the bionematicides at various doses. As a control, a common nematicide was employed. The bionematicide also reduced the number of cysts and the number of female nematodes in the field. Moreover, it also showed that the bionematicide was able to increase the height of potato plants. Bionematicide application also improved various potato growth parameters. The tested formula also increased the number of tubers per plant. The study demonstrated that the most effective and recommended bionematicide concentration was 4% for every 100 mL in each plant. Keywords: Bacillus; endophyte; formulation; Pseudomonas; rhizobacteria. INTRODUCTION Potato (Solanum tuberosum L.) is one of the selected commodities to support the food diversity program to achieve sustainable food security. Potatoes are deemed a staple food because they contain calories, carbohydrates, minerals, and vitamins (Rose and Vasanthakaalam, 2011). In addition, potatoes have a high economic value (Moussa and Solieman, 2016). However, every year potato production in Indonesia is suboptimal due to lower production compared to market demand, even though potatoes are still imported to meet market demand. Commercially, potatoes are highly valuable because potato tubers can be processed into various processed products and used as substitute food ingredients that are healthy and safe for the community (Hussain, 2016). The need for potatoes in Indonesia is increasing every year, which increasingly out-numbers national Submitted: September 05, 2022 Revised: November 12, 2022 Accepted for Publication: December 08, 2022 * Corresponding Author: Email: iisnaza.fkip@unej.ac.id © 2017 Pak. J. Phytopathol. All rights reserved.