Risk in vulnerable groups 959 in the study. The Swedish Medical Birth Register was merged with the Swedish Prescribed Drug Register. The association between offspring’s adult consumption of cardiovascular medicationand i) maternal DM during pregnancy and ii) birth weight were analyzed. Follow up time ranged between 17-36 years. Results: Offspring exposed to maternal DM in utero had an increased risk of non-malformation cardiovascular disease (NMCVD), odds ratio (OR)1.46 (95% confidence interval (CI)1.16-1.83). After further excluding offspring with insulin dependent DM, no increased risk of NMCVD was found, OR 1.19 (95% CI 0.92- 1.55). No increased risk of NMCVD was found in offspring born large for gesta- tional age, OR 1.02 (95% CI 0.96-1.08). An increased risk of NMCVD was found in offspring born small for gestational age, OR 1.29 (95% CI 1.24-1.35). Conclusions: Exposure to maternal DM during pregnancy is not associated with NMCVD in offspring at a maximum of 36 years follow up. Low birth weight is confirmed to be a risk factor for NMCVD while high birth weight is not. P5157 | BEDSIDE Electrocardiographic ST-segment deviations and risk of death: significant age and gender differences in a large primary care population P.V. Rasmussen 1 , J.B. Nielsen 1 , C. Graff 2 , B. Lind 3 , J.J. Struijk 2 , M.S. Olesen 1 , S. Haunsoe 4 , L. Koeber 4 , J.H. Svendsen 4 , A.G. Holst 1 . 1 Copenhagen University Hospital, Department of Cardiology, Laboratory of Molecular Cardiology, Copenhagen, Denmark; 2 Aalborg University, Department of Health Science and Technology, Aalborg, Denmark; 3 Copenhagen General Practitioners’ Laboratory (KPLL), Copenhagen, Denmark; 4 Rigshospitalet - Copenhagen University Hospital, Heart Centre, Department of Cardiology, Copenhagen, Denmark Purpose: ST-segment abnormalities are known to be common, but little more is known about their prevalence and possible prognostic implications. Therefore, we aimed to perform a reference study quantitatively assessing the age- and gender specific consequences of having ST-segment deviations. Methods: We evaluated precordial ST-segment deviations using computerized analysis of ECGs from 301,852 subjects (44.2% men) recorded in a primary care core laboratory. ST-segment deviations were divided into three categories of depression and three of elevation. Separate analyses were performed for men and women above and under the age of 65. Baseline data regarding medication and comorbidity as well as follow-up data were gathered with the use of Danish registries. Our end point was death from cardiovascular disease. Multivariable-adjusted hazard ratios for the differ- ent voltage-categories, in each of the precordial leads, were calculated using Cox P.H. model. All hazard ratios (HR) reported are with reference to an isoelectric ST-segment. Results: After a median follow-up period of 5.8 years, there were 8,282 cardio- vascular deaths (CVD) and 25,574 deaths from non-cardiovascular causes. In general, increasing ST-depression was associated with an increased mortality in a dose-response manner in all precordial leads. This was most pronounced for women <65 years of age in lead V3, where an ST-depression from 100μV to 150μV was associated with a HR of 12.4 (4.6-33.8). ST-elevations in young women were generally associated with increased mortality such as in lead V4, where ST-elevation >150μV was associated with a HR of 10.48 (3.28-33.53). The same elevation in young men was associated with a HR of 0.76 (0.42-1.38). This gender difference was present in all leads except lead V1, where ST- elevation was associated with increased mortality for both men and women. ST- elevation >150μV in V1 was associated with a HR of 2.18 (1.15-4.14) for men >65 years of age and a HR of 2.34 (1.75-3.12) for women >65 years of age. Additionally, ST-elevations in lead V2 and V3 had a certain protective effect in men <65 years. Elevations from 100μV to 150μV in lead V2 was associated with a HR of 0.65 (0.52-0.80) and a HR of 0.76 (0.62-0.92) in lead V3. Conclusion: ST-depression is generally associated with increased mortality in a dose-response manner in all precordial leads and for both genders, with the strongest effect for the young subjects. ST-elevations were associated with in- creased mortality in women but not in men, except in lead V1. We saw a certain protective effect of ST-elevation in lead V2 and V3 for young male subjects. P5158 | BEDSIDE MiR-1, miR-9 and miR-126 levels in peripheral blood mononuclear cells of patients with essential hypertension associate with prognostic indices of ambulatory blood pressure monitoring J.E. Kontaraki 1 , M.E. Marketou 2 , E.A. Zacharis 2 , F.I. Parthenakis 2 , P.E. Vardas 2 . 1 University of Crete, Faculty of Medicine, Molecular Cardiology Laboratory, Heraklion, Greece; 2 University Hospital of Heraklion, Department of Cardiology, Heraklion, Greece Purpose: To assess the expression levels of microRNAs (miRs) implicated in cardiovascular function or disease and possibly playing a role in hypertension in peripheral blood mononuclear cells of patients with essential hypertension and their relationship to target organ damage. Ambulatory blood pressure monitoring (ABPM) is a good predictor of target organ damage in hypertensive patients. We selected to asses the expression levels of miR-1, miR-9 and miR-126. Methods: 24-hour ABPM and blood sampling were performed in 60 untreated participants with essential hypertension (29 males, aged 60.42±9.6 years). Blood samples from 29 healthy individuals (13 males, aged 56.69±8.59 years) were also included for comparison. Peripheral blood mononuclear cells (PBMCs) were isolated and microRNA levels were determined by quantitative real-time reverse transcription PCR. Results: miR-1 (33.94±5.19 versus 12.35±2.13 p=0.006) showed higher lev- els in hypertensive patients compare to healthy control individuals while miR- 9 (6.30±1.10 versus 44.62±15.30, p=0.001) and miR-126 (3.33±0.37 versus 8.15±2.34, p=0.006) showed lower levels in hypertensive patients compare to healthy controls. In hypertensive patients, miR-1 levels showed a significant neg- ative correlation (r=-0.312, p=0.015) with the mean 24-hour dipping status. We also observed significant positive correlations of miR-9 (r=0.300, p=0.021) and miR-126 (r=0.350, p=0.007) levels with the 24-hour mean pulse pressure in hy- pertensive patients. Conclusions: miR-1, miR-9 and miR-126 levels show alterations in PBMCs of hypertensive patients compare to healthy controls and correlate significantly with 24-hour ABPM prognostic indices of target organ damage in hypertensive pa- tients. P5159 | BEDSIDE A newly reclassified fragmented revised cardiac risk index as a prognostic marker in patients undergoing non-cardiac vascular surgery M.H. Bae 1 , W.S. Choi 1 , S.Y. Jang 1 , J.H. Kim 1 , S.H. Park 1 , K.H. Kim 1 , J.H. Lee 2 , H.M. Ryu 2 , D.H. Yang 1 . 1 Kyungpook National University Hospital, Daegu, Korea, Republic of; 2 Gumi Cha Hospital, Gumi, Korea, Republic of Background: There was not known regarding the significance of the fragmented QRS complex (fQRS) in patients undergoing vascular surgery classified as high risk procedure for perioperative cardiac events. The aim of this study was to in- vestigate the value of a newly reclassified fragmented Revised Cardiac Risk Index (RCRI) including the fQRS (fRCRI) as predictor for cardiac events in patients un- dergoing non-cardiac vascular surgery. Methods: A total of 467 consecutive patients (69.4±9.5 years; 403 males) who admitted for non-cardiac vascular surgery were studies. All patients were classi- fied as the RCRI 0, 1, 2, ≥3 group according to the sum of diabetes, renal in- sufficiency, histories of ischemic heart disease, Congestive Heart Failure (CHF), and cerebrovascular disease and then newly reclassified as fRCRI 0, 1, 2, ≥3 group including the fQRS. Major Adverse Cardiac Event (MACE) was defined as a composite of death, Myocardial Infarction (MI), CHF, and percutaneous coro- nary intervention (PCI) before non-cardiac vascular surgery. Results: During index hospitalization, MACE developed in 38 (8.1%) patients (death 3, MI 19, CHF 11, and PCI before vascular surgery 5). The fQRS was present in 169 (36.2%) and it was significantly higher in patients with MACE than in those without MACE (63.2% vs. 34.3%, P<0.001). The incidence of the RCRI 0, 1, 2 and ≥3 was 46.9% (n=219), 35.3% (n=165), 12.4% (n=58), and 5.4% (n=25), respectively. When the data for the fRCRI including the fQRS were ana- lyzed, 28 patients (48.3%) belonged to the RCRI 2 were reclassified as the fRCRI ≥3 (Figure 1). In multivariate logistic regression analysis, fRCRI (Odds ratio [OR] 1.529, 95% confidence interval [CI] 1.035-2.258, P=0.033) in addition to left ven- tricular ejection fraction <50% (OR 2.679, 95% CI 1.102-6.511, P=0.030) was an independent predictor for in-hospital MACE after adjusting for age ≥70 years, current smoking, ST-T wave change, and left ventricular hypertrophy on ECG. Conclusion: A newly reclassified fRCRI including the fQRS is an indepen- dent predictor for in-hospital MACE in patients undergoing non-cardiac vascular surgery P5160 | BEDSIDE Impact of positive airway pressure treatment in non-obese patients with cardiovascular disease and sleep-disordered breathing Y.Nishihata, Y. Takata, K. Kato, T. Yamaguchi, K. Shiina,Y. Usui, A.Yamashina. Tokyo Medical University, Tokyo, Japan Purpose: We assessed whether sleep-disordered breathing (SDB) is a risk fac- tor for poor prognosis in non-obese patients with cardiovascular disease (CVD) and whether positive airway pressure (PAP) treatment improves cardiovascular outcomes. Methods: We investigated 1693 patients who underwent a polysomnography, and enrolled consecutive 142 non-obese patients (body mass index ≤ 25), who had been hospitalized due to CVD before the sleep study. They were divided into 3 groups; a non-to-mild SDB group (apnea-hypopnea index [AHI] < 15/h), an untreated SDB group (AHI ≥ 15/h and refused PAP treatment initially), and a PAP-treated group (AHI ≥ 15/h and treated with PAP). The remaining 13 patients were excluded from data analyses because they had discontinued PAP treatment. The frequency of death and re-hospitalization due to cardiovascular events (acute coronary syndrome, coronary revascularization, heart failure, stroke, arrhythmias, and aortic dissection) among the groups was analyzed using multivariate analy- sis. Results: The mean follow-up period was 30.7±23.6 months and 37 patients (28.7%) had cardiovascular events. Kaplan-Meier survival curves indicated that event-free survival was significantly lower in the untreated SDB group than in the other groups (Figure). Multivariate analysis showed that the risk for CVD events Downloaded from https://academic.oup.com/eurheartj/article-abstract/34/suppl_1/P5158/2863072 by guest on 07 June 2020