MAIN ©1996-2017 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page OJVRTM Online Journal of Veterinary Research© Volume 21(7):387-410, 2017. Effect of Holothuria spp fish tissue extracts on A. saline shrimp brine and human cancer MCF-7 and HeLa cells. Sakineh Mashjoor, Morteza Yousefzadi Department of Marine biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran A B S T R A C T Mashjoor S, Yousefzadi M., Effect of Holothuria spp fish tissue extracts on A. saline shrimp brine and human cancer MCF-7 and HeLa cells. OnlJ Vet Res., 21(7):387-410, 2017. Effect of n-Hexane, ethyl acetate and methanol Holothuria scabra, Holothuria parva and Holothuria leucospilota extracts on viability of A saline shrimp larvae and MCF-7-HeLa cancer cells is described. In shrimp assay, cuvierian tubules of H. leucospilota in methanol extract induced 95% mortality at 1000 µg/ml with an IC 50 = 616.4 μg/ml and respiratory tree organs of H.parva, 86% mortality at 1000 µg/ml with an IC 50 of 607.2 μg/ml. In cancer MCF-7 cells, E fractions of coelomic fluid from H. leucospilota killed 85% cells at 250 µg/ml with an IC 50 = 37.25 μg/ml and in HeLa cancer cells, E extracts of intestine tract organs of H.parva killed 80% at 250 µg/ml, IC 50 = 46.25 μg/ml. We find that methanolic extracts of Holothuria spp had a significant cytotoxic effect on A. saline larvae but not on the cancer cell lines used in this study. Keywords: Sea cucumber; Cell line; Biological activities; Secondary metabolites; Persian Gulf. INTRODUCTION Sea cucumber marine invertebrates of the phylum Echinoderm are found in benthic areas and deep sea floors worldwide (Althunibat et al., 2009). Tissues from these fish may have anticancer, anti-proliferative (Roginsky et al., 2004; Soltani and Baharara, 2014; Wang et al., 2014; Seydi et al., 2015; Assawasuparerk et al., 2016), anti-angiogenic (Tian et al., 2005), antimicrobial (Mokhlesi et al., 2012; Mohammadizadeh