国立科博専報, (50), pp.21–28, 2014 年 3 月 28 日 Mem. Natl. Mus. Nat. Sci., Tokyo, (50), pp.21–28, March 28, 2014 Testate Amoebae of the Imperial Palace, Tokyo Satoshi Shimano 1* , Anatoly Bobrov 2 and Yuri Mazei 3 1 Environmental Education Center, Miyagi University of Education, Aramaki Aza-Aoba, Aoba-ku, Sendai city, Miyagi, 980–0845 Japan. *mitesproto@gmail.com 2 Lomonosov Moscow State University, Leninskie gory, Moscow, Russia 3 Penza State University, Penza, Russia Abstract. The testate amoeba fauna of the Imperial Palace, Tokyo was surveyed. Totally 54 species, 12 varieties and forms belonging to 14 genera and two taxa with unknown taxonomic position of testate amoebae were record- ed there. One genus, Planhoogenraadia, 19 species and 11 varieties and forms are newly recorded from Japan. Testate amoebae community in Imperial Palace is characterized by low alpha-diversity and high beta-diversity. Most common taxa, found in more than half of the sampling sites, are Cyclopyxis eurystoma v. parvula, Euglypha laevis and Trinema lineare. Key words: Amoebozoa, Imperial Palace, new record in Japan, Rizaria, soil, suspended litter, testate amoebae, Introduction Testate amoebae (belonged to supergroups Amoebozoa and Rhizaria: Adl et al., 2012) from ter- restrial habitats in Japan have been inadequately stud- ied. Soil protists of Japan have not been reviewed but Shimano & Miyoshi (2008) summarized publications on ciliates and testate amoebae as a bibliography. The few existing data on soil testate amoebae in Japan are of special interest for biogeography and ecology. Publications dealing with specific aspects of morphology and biology of soil testate amoeba are scarce (e.g. Aoki (2003) and Aoki et al. (2007)). Coûteaux (1978) described several new species and one new genus found on the island of Honshu, in the valley of Shiga, 200 kilometers northwest of Tokyo. Recently, Bobrov et al. (2012) described two new species of testate amoebae from mountain massif Tateyama, Toyama prefecture, central Japan. In the Imperial Palace, some prominent testate amoebae species were recorded from fresh water habitats. Tanaka & Takeda (2000) identified 21 species, Arcella catinus Pénard, 1890; Arcella conica (Playfair, 1918) Deflandre, 1926; Arcella discoides Ehrenberg, 1871; Arcella polypora Pénard, 1890; Arcella vulgaris Ehrenberg, 1832; Centropyxis aculeata (Ehrenberg, 1830) Stein, 1857; Centropyxis hirsuta Deflandre, 1929; Centropyxis discoides (Pénard, 1890) Deflandre, 1929; Centropyxis spinosa (Cash, 1905) Deflandre, 1929; Lesquereusia modesta Rhumbler, 1895; Nebela tincta (Leidy, 1879) Awerintzew, 1906; Difflugia acuminata Ehrenberg, 1838; Difflugia tuberculata (Wallich, 1864) Archer, 1867; Difflugia avellana Pénard, 1890; Difflugia globulosa Dujardin, 1837; Difflugia gramen Pénard, 1902; Difflugia labiosa Wailes, 1919; Difflugia curvicaulis Pénard, 1899; Difflugia lanceolata Pénard, 1890; Difflugia corona Wallich, 1864; Euglypha filifera Pénard, 1890. Tanaka et al. (2006) also recorded five species, Arcella dentata Ehrenberg, 1832; Difflugia oblonga Ehrenberg, 1838; Heleopera sphagni (Leidy, 1874) Hopkinson, 1909; Trinema lineare Pénard, 1890; Trinema enchelys (Ehrenberg, 1838) Leidy, 1878. Thus, totally 25 species (taking into the account that Difflugia curvicaulis is the junior synonym of Difflugia acuminata: see Mazei & Warren, 2012) were recorded from the Imperial Palace. In present study, some samples were taken from several habitats, mainly from soil.