198 (Continued on next page) GROWTH PERFORMANCE AND QUALITY TRAITS OF SIBERIAN STURGEON A. baerii JUVENILES FED DIETS INCLUDING Nannochloropsis gaditana AND Scenedesmus almeriensis MICROALGAE MEAL T. Bongiorno* 1 , A. Di Biase 2 , M. Vasconi 3 , V.M. Moretti 3 , A. Lopez 3 , A. Tava 4 , D. Carminati 4 , G. D’Imporzano 5 , M. Montedoro 1 , F. Adani 5 , F. G. Acién Fernández 6 , F. J. Alarcón 7 , K. Parati 1 1 Istituto Sperimentale Italiano L. Spallanzani - Loc. La Quercia, 26027 Rivolta d’Adda (CR), Italy 2 AIA-Agricola Italiana Alimentare, Spa, Brand Veronesi Mangimi, Verona, Italy 3 Dipartimento di Scienze Veterinarie, Università degli Studi di Milano, Italy 4 Centro di ricerca Zootecnia e Acquacoltura, CREA-ZA (LO), Italy 5 Università degli Studi di Milano, Dipartimento di Scienze Ambientali e Agraria (DiSAA) Milano, Italy 6 Department of Chemical Engineering, University of Almerıa, 04120 Almerıa, Spain 7 Department of Biology and Geology, University of Almerıa, 04120 Almería, Spain E-mail: tiziana.bongiorno@istitutospallanzani.it Introduction The demand for aquafeed grown exponentially in the last decade and is expected to increase further in the coming years (FAO 2018). The wild fish used for the fish meal and oil, currently used in feed formulated for carnivorous species, will be used in human consumption and less available for aquaculture. Among the potential ingredients of future use in aquafeed, microalgae represent a promising matrix, as characterized by nutritional, nutraceuticals and immunostimulant properties (Camacho-Rodríguez et al., 2017). However the high production cost is a limiting factor for their use. The development of a microalgae-based biorefiner , able to use some by-products and agro-industrial waste to produce biomass would enable to limit the disposal costs sustained by the companies and to lower the production costs of the microalgae. The present research was undertaken to evaluate effect on growth response and fillet quality traits of sturgeon (A. baerii) fed with two microalgae freeze-dried biomass Nannochloropsis gaditana and Scenedesmus almeriensis grown in Sinthetic Medium (SM) or in diluted Pig Manure (PM) and included in partial substitution of dietary fish meal and oil Materials and methods Four complete diets were formulated to be grossly iso-proteic and iso-lipidic. A control diet (C) was prepared using a blend of conventional animal and vegetal protein sources. The test diets coined respectively N. gaditana grown on Synthetic Medium (NSM), N. gaditana grownoin pig manure (NPM), S. almeriensis grown on Synthetic Medium (SSM) and S. almeriensis grown on Pig Manure (SPM) were prepared by replacing the 10% of protein and lipid supplied by the blend of conventional protein and lipid-rich ingredients with microalgae. All the ingredients are mixed and pelleted by a cold extrusion process (70°C). Each diet were randomly assigned to tank and tested in triplicate according to a monofactorial design. Microalgae dried biomass and diets were analized microbiologically and verified for nutritional quality. To carried out the feeding trials 240 juvenile A. baerii (average 12.8±0.3g each) were randomly allocated among 15 circular tanks (16 fish/tank) in RAS system under controlled rearing conditions (temperature, 19°C, DO 9.6 mg/L, artificial daylenght, 12h). Diets were offered in two daily meals with a fixed feed ratio (3% body mass) over 6 weeks and each group were weighted every week under moderate anaesthesia. At the end of the trial, survival rate (%), Final Body Weight (FBW), Specific Growth Rate (SGR), Feed Conversion Ratio (FCR), Feed Intake (FI), were evaluated. Furthermore, nine fish per feed treatment were analized to determine the biometric indeces, fillet proximate composition and oxidation parameters (SOD, CAT, GPX, 8-isoprostanes). Data were subjected to ANOVA and differences tested by the Tukey’s test (P < 0.05). brought to you by CORE View metadata, citation and similar papers at core.ac.uk provided by AIR Universita degli studi di Milano