*Correspondence: sukhendu1@hotmail.com (Received: July 31, 2021; accepted: December 16, 2021) Citaton: Uddin MR, Roy P, Mandal S. Culturable Bacterial Isolates from Arctc Soil shows High Biotechnological Potental. J Pure Appl Microbiol. 2022;16(1):235-245. doi: 10.22207/JPAM.16.1.13 © The Author(s) 2022. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Uddin et al. | J Pure Appl Microbiol | 16(1):235-245 | March 2022 Artcle 7211 | htps://doi.org/10.22207/JPAM.16.1.13 Published Online January 10, 2022 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle OPeN ACCess www.microbiologyjournal.org 235 Journal of Pure and Applied Microbiology Culturable Bacterial Isolates from Arctc Soil shows High Biotechnological Potental Md. Raihan Uddin 1 , Pranab Roy 2 and Sukhendu Mandal 1 * 1 Department of Microbiology, Laboratory of Molecular Bacteriology, University of Calcuta, 35, Ballygunge Circular Road, Kolkata - 700 019, West Bengal, India. 2 Department of Molecular Biology, Insttute of Child Health, 11, Dr. Biresh Guha Street, Kolkata - 700 017, West Bengal, India. Abstract Polar microbiology remains as the most fascinatng area of research which mainly focuses on exploraton of psychrophilic organisms for having their cold-actve enzymes of biotechnological potental. In this study, we have explored a culturable bacterial community and isolated 27 bacterial isolates with a diferent morphology from an unexplored site of Arctc region, for the possibility of identfying various actve biomolecules. Screening of various isolates in a culture dependent manner helped us to identfy strains capable of producing extracellular enzymes. The optmal growth parameters of most of the isolates are ranges between 18-22°C temperature, 3-5 days of incubaton, 6-9 pH, and 3-5% (w/v) NaCl in LB media. It has also been found that among these isolates, 63% are able to produce lipase, 17% amylase, 7% xylanase and 7% isolates have responded for phosphatase actvity but there are no isolates found for gelatnase and cellulase producton ability. In additon, few isolates can also produce secretory protease, urease, β-galactosidase, etc. 16SrRNA gene sequence-based phylogeny revealed that the isolates belong to the genera of Psychrobacter, Planococcus, Halomonas, Arthrobacter, Oceanisphaera, Marinbacter, Pseudomonas, Algoriphagus. Strikingly, none of the Arctc isolates showed resistance towards commonly used antbiotcs which indicates that the unexplored habitat is devoid of antbiotc exposure and so does the rise of antmicrobial resistance. The structure-functon relatonship of the isolated bioactve compounds from these isolates are the major focus of future research. Keywords: Psychrotrophs, Arctc, Community Analysis, Polar Microbiology, Lipase, Pigment, Cold-actve enzymes