Jurnal Kedokteran Hewan March 2023, 17(1):10-13 P-ISSN : 1978-225X; E-ISSN : 2502-5600 DOI: https://doi.org/10.21157/j.ked.hewan.v17i1. 29785 17 Revised 21-02-2023, Accepted 27-02-2023 EVALUATION OF LAPAROTOMY SURGERY WOUND HEALING IN DOGS USING THERMOGRAPHIC ANALYSIS Agustina Dwi Wijayanti 1 *, Agus Purnomo 2 , and Hery Wijayanto 2 1 Study Program in Biomedical Engineering, Sekolah Pasca Sarjana Universitas Gadjah Mada, Indonesia 2 Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia *Corresponding author: tinabdy@ugm.ac.id ABSTRACT This study aimed to develop the use of thermographic analysis to evaluate wound healing for laparotomy surgery in animals. The experiment used nine adult dogs 5 males and 4 females, bodyweight 5-10 kg) that had undergone the same general anesthesia and laparotomy procedures. Evaluation of wound healing was performed at 24, 48, and 72 hours after surgery and 7 days after operations using a digital thermal camera. During each thermographic evaluation period, blood samples were taken for analysis of total leukocytes and leukocyte differential. The results of thermal imaging were compared with the values of inflammatory cells and the clinical condition of wound healing. Comparison of thermographic analysis with inflammatory status was evaluated using the regression equation and showed a strong correlation coefficient (Y= -0.4847 + 40.14, R 2 = 0.867), that is, the higher the temperature, the lower the inflammation. The conclusion of study is that thermographic analysis can be used to evaluate wound healing of laparotomy surgery in dogs. ____________________________________________________________________________________________________________________ Key words: dog, laparotomy, thermal camera, thermography, wound healing ABSTRAK Penelitian bertujuan mengembangkan penggunaan analisis termografi untuk mengevaluasi kesembuhan luka operasi laparatomi pada hewan. Penelitian menggunakan sembilan ekor anjing dewasa (5 ekor jantan dan 4 ekor betina, berat badan 5-10 kg) yang telah menjalani prosedur anestesi umum dan operasi laparatomi yang sama. Evaluasi kesembuhan luka dilakukan pada jam ke-24, 48, 72 jam setelah operasi dan padahari ke-7 setelah operasi menggunakan kamera termal digital. Pada setiap periode evaluasi termografi, darah diambil untuk analisis leukosit total dan diferensial leukosit. Hasil pencitraan termal dibandingkan dengan nilai sel-sel radang dan kondisi klinis kesembuhan luka. Perbandingan analisis termografi dengan status keradangan dievaluasi menggunakan persamaan regresi. Hasil menunjukkan koefisien korelasi yang kuat (Y= -0,4847+ 40,14, R 2 = 0,867), yaitu semakin tinggi suhu, semakin turun keradangan. Kesimpulan penelitian adalah analisis termografi dapat dimanfaatkan untuk evaluasi kesembuhan luka operasi laparatomi pada anjing. ____________________________________________________________________________________________________________________ Kata kunci: anjing, laparatomi, kamera thermal, termografi, kesembuhan luka INTRODUCTION Infrared Thermography (IRT) is a new diagnostic technology that has not been widely used in the veterinary field. This technique has received great attention in medicine and industry as a non-invasive method that does not use radiation (Rocha and Póvoas 2017). Thermography is used to diagnose several disorders of the body, including vascular, neurological, orthopedic, and neoplastic diseases, or other conditions that involve changes in body surface temperature. An increase in body temperature is associated with an increase in local circulation and metabolism, which can be clinically correlated with inflammation; whereas a decrease in body temperature is clinically associated with decreased tissue perfusion due to infarction or changes in the autonomic nervous system (Grossbard et al. 2014). The IRT technique detects the radiation spectrum of an object using a camera that produces a thermal image which is visualized and interpreted using special software. Infrared thermography makes it possible to visualize temperature changes on the surface of the skin which can be correlated with the organs of the body being evaluated, for example, in cases of osteoarthritis, spinal cord injury, conducting evaluations, infectious diseases, and malignant neoplasia. In contrast to existing clinical methodologies, IRT has the advantage of being non-invasive for assessing the thermal state of animals without the need for physical examination or chemical restraint. This technology is considered a non-contact attachment technique that does not require attaching a device to the animal's body and may be a useful method for avoiding hyperthermia induced by the stress of handling. Hildebrandt et al. (2010) observed that several types of local tissue lesions are associated with variations in blood flow that affect surface temperature, such as inflammation leading to hyperthermia. The distribution of heat in the thermography method indicates the dynamics of inflammation in the tissue, and can be evaluated continuously during the healing process. This technique is without contact, non-invasive and fast (Ireneusz et al. 2015). The use of effective and non- invasive IRT technology is also in line with animal welfare which emphasizes the rights of animals not to be hurt and their freedom disturbed. This study aims to utilize IRT with a digital thermal camera to evaluate wound healing during laparotomy surgery in dogs. As a comparison, clinical observation of the wound and blood sampling will be carried out to evaluate inflammatory cells together with the thermographic evaluation. MATERIALS AND METHODS The research was conducted at the Prof Soeparwi Animal Hospital, Karangmalang, Yogyakarta, from September to November 2022. This research has received ethical approval from the Ethics Commission