Jour nal Ho me-Page: http://vetdergi.kafkas.edu.tr Onl ine Submission : http://vetdergikafkas.org Evaluation of Bd-2, Bd-XL and Bax Expression and Apoptotic Index in Canine Mammary Tumours[1] Funda YILDIRIMKivilcim SONMEZ 1 __ Hande OZYOGURTQU 1 Gulbin §ENNAZLI1 Aydin GUREL 1 Mehmet Can GUNDUZ2 Besim Hasan SONTA§ 2 This work was supported by Istanbul University Scientific Research Projects Department (Project Number: NP-1070, UDP-35787) 2 lstanbul University, Faculty of Veterinary Medicine, Pathology Department, TR-34320 Avcilar, Istanbul - TURKEY Istanbul University, Faculty of Veterinary Medicine, Gynecology and Obstetrics Department, TR-34320 Avcilar Istanbul-TURKEY RESEARCH ARTICLE Kafkas Univ Vet Fak Derg 20 (4): 513-520, 2014 DPI: 10.9775/kvfd.2013.10450 Makale Kodu (Article Code): KVFD-2013-10450 Summary Mammary tumours are the most common neoplasms in intact female dogs. Dysregulation of programmed cell death mechanisms plays an important role in the pathogenesis and progression of mammary gland tumours. The aim of this study was to investigate the relationship between some anti-apoptotic proteins (Bcl-2, Bcl-XL and Bax), apoptotic index (Al) and histopathological diagnosis, tumour grading, tumour staging and survival time of canine mammary tumours (CMT). Twenty seven tissue samples were collected from twenty seven animals with mammary tumours. The samples were evaluated and graded histopathologically. All cases were staged according to the TNM system. The expression of Bcl-2, Bcl-XL and Bax proteins was investigated using indirect immunoperoxidase test and apoptosis was evaluated usinq terminal deoxynucleotidyltransferase (TdT)-mediated nick end-labelling (TUNEL) technique. Follow-up examination and survival estimation analysis were performed. While there was a significant statistical relation between Bcl-2 expression and histopathological diagnosis (P<0.005), there was no considerable association between histopathological diagnosis and Bax, Bcl-XL and Al (P>0.05).The differences between T1 a n d T ^ andT5 stages were statistically significant in terms of Bax expression (P<0.05), and Bax expressions were higher in T5 when compared with T1 or T2 No association between survival time and Bcl-2, Bax, Bcl-XL and Al was determined (P>0.05). Bcl-2 was overexpressed in highly malignant tumours such as solid and tubulopapillary adenocarcinomas and Bax had high expression levels in metastatic tumours. As a result, it is concluded that Bcl-2 and Bax expression can be accessory parameters for anticipating the biologic behaviour and prognosis of CMT but these markers alone are not sufficient for the determination of survival time. Keywords: Canine mammary tumour, Apoptosis, Bcl-2, Bci-XL , Bax, immunohistochemistry, TUNEL Kopek Meme Tumorlerinde Bd-2, Bcl-XL ve Bax Sunulumu ile Apoptotik indeksin Degerlendirilmesi Ozet Meme tumbrleri intakt di§i kbpeklerin en sik kar§ila5ilan tumbrleridir. Programl, hucre blurn mekanizmalarmda meydana gelen diizensizlikler meme bezi tumorlermin patogenezis ve progresyonunda bnemli bir rol oynamaktadir. Bu ?ali5manm amaci kopek meme tumorlerinde (KMT) bazi anti-apoptotik proteinler (Bcl-2, Bcl-XL and Bax) ve apoptotik indeksin (Al), histopatolojik tarn, tumor derecelendirmesi, tumor evreleri ve kaian ya$am sureleri lie ili5kisini ortaya koymaktir. Meme tumbru olan yirmi yedi hayvandan yirmi yedi biyopsi brnegi toplandi. Ornekler histopatolojik olarak incelendi ve derecelendirildi. Butun olguiarTNM sistemine gore evrelendi. Bcl-2, Bcl-XL ve Bax proteinlerinin sunulumu indirect immunoperoksidaz test! ile ve apoptozis ise terminal deoksinukleotidiltransferaz (TdT)-aracili nick end-labelling (TUNEL) tekniqi ile mcelend1. Hasta takibi ve hayatta kalma suresi tahmini analizleri ger?ekle5tirildi. Bcl-2 sunulumu ve histopatolojik tarn arasinda bnemli bir istatistiksel ili5ki belirlemrken (P<0.005), histopatolojik tarn ile Bax, Bcl-XL ve Al arasinda dikkate deger bir ilijki saptanmadi (P>0 05) Bax sunulumu agsindanTI veT5 ileT2 veT5 evreleri arasinda bnemli istatistiksel farkhhklar belirlendi (P<0.05) veTI veya T2 ile karjilastinldiqmda Bax sunu'umu T5te daha yuksekti. Hayatta kalma suresi ile Bcl-2, Bax, Bcl-XL sunulumlan ve Al arasinda hi^bir ili5ki saptanmadi (P>0 05) Bd-2 nm solid ve tubulopapiller adenokarsinomalar gibi malignitesi yuksek tCimbrlerde a$in diizeylerde sunuldugu ve Bax'm metastazik tumorlerde sunuluniunun yuksek oldugu belirlendi. Sonu? olarak, Bcl-2 ve Bax sunulumlan KMT'nin biyolojik davram5 ve prognozlarmi tahmin etmede yardimci parametreler olabilecegi fakat bu belirte?lerin tek ba5larina hayatta kalma suresinin belirlenmesi igin yeterli olmadigma karar verildi. v 7 Anahtar sozcukler: Kopek, Meme tumoru, Apoptozis, Bcl-2, Bcl-XL , Bax, immunohistokimya, TUNEL A? iletijim (Correspondence) ® +212 4737070/17094 El funda@istanbul.edu.tr