Research Article FacileandEco-FriendlyFabricationofSilverNanoparticlesUsing Nyctanthes arbor-tristis Leaf Extract to Study Antibiofilm and Anticancer Properties against Candida albicans Beema Shafreen Rajamohamed , 1,2 Seema Siddharthan , 2 Velmurugan Palanivel , 3 Mohanavel Vinayagam , 3 Vijayanand Selvaraj , 4 Sivakumar Subpiramaniyam , 5 SalehH.Salmen , 6 SamiAlObaid, 6 SekarPalanivel, 7 andSenthilkumarSubramanian 8 1 Department of Biotechnology, Dr. Umayal Ramanathan College for Women, Algappapuram, Karaikudi 630003, India 2 Molecular and Nanomedicine Research Unit, Centre for Nanoscience and Nanotechnology (CNSNT), Sathyabama Institute of Science and Technology, Chennai 600119, TN, India 3 Centre for Materials Engineering and Regenerative Medicine, Bharath Institute of Higher Education and Research, Selaiyur, Chennai, Tamilnadu, India 4 Department of Biotechnology, iruvalluvar University, Serkadu, Tamilnadu, India 5 Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University, Miryang 50463, Republic of Korea 6 Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia 7 Department of Zoology, Arignar Anna Government Arts College, Namakkal, Tamil Nadu, India 8 College of Medicine and Health Science, Jigjiga University, P.O. Box 1020, Jigjiga, Somali Region, Ethiopia Correspondence should be addressed to Velmurugan Palanivel; palanivelmurugan2008@gmail.com and Senthilkumar Subramanian; senthilkumarsubramanian@jju.edu.et Received 27 September 2021; Revised 7 December 2021; Accepted 14 December 2021; Published 7 January 2022 Academic Editor: Esmaeel Sharifi Copyright © 2022 Beema Shafreen Rajamohamed et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. e synthesis of silver nanoparticles has been gaining more attention in recent years due to their small size and high stability. For this study, silver nanoparticles were biosynthesized from leaf extract of the medicinal plant (N. arbor-tristis). Vitally, the shrub with tremendous medicinal usage was diversely observed in South Asia and South East Asia. e synthesized silver nanoparticles were characterized by color visualization, ultraviolet-visible spectrophotometry (UV-Vis), Fourier-transform infrared spec- troscopy (FTIR), field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS) technique. A sharp peak at 427 nm for biosynthesized nanoparticles was obtained using UV-Vis, which represents surface plasmon resonance. us, characterization techniques showed the green synthesis of AgNPs leads to the fabrication of spherical shape particles with a size of 67 nm. Furthermore, AgNPs were subjected to antibiofilm studies against Candida albicans and it was observed that 0.5 μgmL −1 of AgNPs significantly reduced 50% of biofilm formation. ese bio- synthesized nanoparticles also showed a considerable reduction in viability of HeLa cells at 0.5 μgmL −1 . e morphological changes induced by AgNPs were observed by AO/EB staining. e toxic effect of AgNPs was studied using brine shrimp as a model system. erefore, it is envisaged that further investigation with these AgNPs can replace toxic chemicals, assist in the development of biomedical implants that can prevent biofilm formation, and avoid infections due to C. albicans. Hindawi Advances in Materials Science and Engineering Volume 2022, Article ID 2509089, 10 pages https://doi.org/10.1155/2022/2509089