_____________________________________________________________________________________________________ *Corresponding author: E-mail: princemakat@yahoo.co.uk; Microbiology Research Journal International 18(4): 1-10, 2017; Article no.MRJI.30857 Previously known as British Microbiology Research Journal ISSN: 2231-0886, NLM ID: 101608140 SCIENCEDOMAIN international www.sciencedomain.org Impact of Rigwash Oil Spill ‘Dispersants’ on the Biodegradation of Crude Oil in Nigeria Prince C. Nnadozie 1* and Lucky O. Odokuma 1 1 Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, P.M.B. 5323, Choba, Rivers State, Nigeria. Authors’ contributions This work was carried out in collaboration between both authors. Author PCN designed the study, performed the statistical analysis, wrote the protocol and the manuscript, managed the analyses of the study and the literature searches under the strict supervision of author LOO. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/MRJI/2017/30857 Editor(s): (1) Marcin Lukaszewicz, Department of Biotransformation, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland and Division of Chemistry and Technology Fuels, Wroclaw University of Technology, Wroclaw, Poland. (2) Tetsuya Akitsu, University of Yamanashi, Japan. Reviewers: (1) Nwankwegu Amechi Sampson, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria. (2) Khusnu Yaqin, Hasanuddin University, Indonesia. Complete Peer review History: http://www.sciencedomain.org/review-history/17765 Received 3 rd December 2016 Accepted 2 nd February 2017 Published 8 th February 2017 ABSTRACT Aim: To ascertain the impact of Rigwash oil spill ‘dispersant’ on the biodegradation of crude oil. Study Design: The study employs experimental design and statistical analysis of data and interpretation. Place and Duration of Study: The water sample for the study was collected from Okrika High Sea via Adedemebiri, Rivers State of Nigeria at a depth of 1 m. It was transported to the laboratory at 25 – 30°C within 2 hours. The ‘dispersant’ and the o il were obtained from an industrial chemical store at Trans-Amadi, Port Harcourt and from the Nigerian National Petroleum Corporation (NNPC), Port Harcourt, Rivers State of Nigeria respectively. Experimental analyses were done at the Departments of Microbiology, and Biochemistry, University of Port Harcourt, Rivers State, and at the Federal Institute of Industrial Research, Oshodi (FIIRO), Lagos State. The biodegradation setups were monitored at a weekly interval over 21-days period at room temperature (approximately 30°C) on a static shake-flask system. Methodology: Standard techniques such as the spread plate method, titrimetric technique, atomic Original Research Article