_____________________________________________________________________________________________________ *Corresponding author: E-mail: saad_rehman101@hotmail.com; Asian Journal of Biochemistry, Genetics and Molecular Biology 10(4): 1-26, 2022; Article no.AJBGMB.85337 ISSN: 2582-3698 Tapping into the Unsung Potential of CRISPR/CAS Technology in Agriculture Rao Saad Rehman a* , Mujahid Ali b , Syed Ali Zafar c , Muhammad Ahmad b , Asad Nadeem Pasha d , Hassan Bashir e , Faiza Rashid e and Mubashar Hussain a a College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China. b Department of Plant Breeding and Genetics, Nanjing Agricultural University, Nanjing, China. c Oilseeds Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan. d Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan. e Department of Plant Breeding and Genetics, Sub Campus Burewala-Vehari, University of Agriculture Faisalabad, Pakistan. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/AJBGMB/2022/v10i430249 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/85337 Received 22 January 2022 Accepted 30 March 2022 Published 04 April 2022 ABSTRACT Over the last few years, the use of clustered regularly interspaced short palindromic repeats (CRISPR) for genetic manipulation has transformed life science. CRISPR was first found in bacteria and archaea as an adaptable immune system, and later modified to create specific DNA breaks in living cells and creatures. Various DNA alterations can occur throughout the cellular DNA repair process. Since the first demonstration of CRISPR in plant genome editing in 2013, there has been much progress in fundamental crop research and plant improvement. Plants can use the CRISPR toolset to do programmable genome editing, epigenome editing, and transcriptome regulation. However, the difficulties of plant genome editing must be properly understood and answers sought. With an emphasis on achievements and prospective utility in plant biology, this review aims to provide an instructive assessment of the current advancements and discoveries in CRISPR technology. CRISPR will, in the end, not only make fundamental research easier, but it will also speed up plant breeding and germplasm development. In the light of global climate change, as well as present agricultural, environmental, and ecological concerns, the use of CRISPR to improve germplasm is extremely significant. Review Article