Interna RP-HPLC METHOD D EXPERIMENTAL DESI ESTIMATION OF THIOC FORCED DEGRADATION Dr. Alisha Patel* 1 , Dr. Biren Sha 1,4 ROFEL Shri G. M. Bilakhia College of 2 Shree Naranjibhai Lalabhai Patel Colleg 3 B.J.Medical college (Department of Orth Correspondence Address: Dr. Alisha P Vapi, Gujarat, India, E-mail : alishabhm4 __________________________________ ABSTRACT Introduction: In the present w quantitative estimation of Thiocol form. Material & methods: Determin (250×4.6mm, 5μ) column using a (70:30 v/v) pH 4.5, Flow rate of 1. Result: The linearity was found (r 2 =0.9991, and r 2 =0.9992) for TH clear baseline separation with a respectively. Discussion: The forced degradatio thermal conditions at different tim Conference on Harmonization (IC Quality by Design approach used of Designs the three chromatograp were changed and study were carri Conclusion: The proposed validat commercially available dosage for ____________________________ Key words: Thiocolchicoside, Dex INTRODUCTION Thiocolchicoside, Chemically N (methylsulfanyl) -9 -oxo -5,6,7,9 with anti-inflammatory and analg glucoside, derivative of colchicine IJARR, 1(6 178 ational Journal of Advanced Research a www.ijarr.in DEVELOPMENT AND VALIDATI IGN APPROACH FOR SIMU COLCHICOSIDE AND DEXKETOPRO N STUDIES ah 2 , Dr. Keyur Patel, Dr. Arindam Paul, Dr. R f Pharmacy, Vapi, Gujarat, India ge of Pharmacy, Surat, Gujarat, India hopedics), Ahmedabad, Gujarat, India Patel, ROFEL Shri G. M. Bilakhia College of Pharmacy 4@gmail.com ______________________________________________ work, sensitive RP-HPLC method been dev lchicoside (THC) and Dexketoprofen (DKP) c nation of THC and DKP was carried on a re mobile phase consisting of Methanol: Sodium P .0 ml/min and the detection was carried out at 28 d to be in the range of 5-40 g/ml and 30- HC and DKP respectively. The sharp peaks obtai retention time of 3.20 min and 8.40 min for on studies performed in acidic, basic, oxidative me intervals. The method was validated as per CH) guidelines as well as for the ROBUSTN based on 3-Level Factorial Design of Experim phic parameters (Flow Rate, pH and Mobile pha ied out with effects on Retention time and Peak ted method was successfully used for the quanti rm. _______________________________________ xketoprofen, QbD based RP-HPLC, Factorial de - [ (7S) -3- (beta-D-glucopyranosyloxy) -1, 2- -tetrahydrobenzo [a] heptalen-7-yl] acetamide. gesic activity. Thiocolchicoside is a 3-demeth es. It acts as a competitive GABA A receptor an 6), 2016; 178-195 and Review ION USING ULTANEOUS ROFEN WITH Rajvi Desai y, Namdha Campus, _________________ veloped for the combined dosage everse phase C 18 Phosphate Buffer 80 nm. -240 g/ml with ined were having r THC and DKP e, photolytic and the International NESS studies the ment. On the basis hase composition) Area. itative analysis of ______________ esign - dimethoxy- 10- Muscle relaxant hyl-thiocolchicine ntagonist and also