Accepted Manuscript Title: Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFN2 signal peptide Author: Ram´ on Rom´ an Joan Miret Federica Scalia Antoni Casablancas Mart´ ı Lecina Jordi J. Cair ´ o PII: S0168-1656(16)31554-1 DOI: http://dx.doi.org/doi:10.1016/j.jbiotec.2016.10.005 Reference: BIOTEC 7684 To appear in: Journal of Biotechnology Received date: 12-7-2016 Revised date: 3-10-2016 Accepted date: 6-10-2016 Please cite this article as: Rom´ an, Ram´ on, Miret, Joan, Scalia, Federica, Casablancas, Antoni, Lecina, Mart´ ı, Cair´ o, Jordi J., Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFN2 signal peptide.Journal of Biotechnology http://dx.doi.org/10.1016/j.jbiotec.2016.10.005 This is a PDF ﬁle of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its ﬁnal form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.