Vol.:(0123456789) 1 3
Metabolomics ( 2019) 15:141
https://doi.org/10.1007/s11306-019-1593-3
ORIGINAL ARTICLE
Post‑periodontal surgery propounds early repair salivary biomarkers
by
1
H NMR based metabolomics
Manvendra Pratap Singh
1
· Mona Saxena
2,4
· Charanjit S. Saimbi
2
· Mohammed Haris Siddiqui
3
· Raja Roy
1
Received: 24 January 2019 / Accepted: 25 September 2019
© Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract
Introduction Oral microfora is a well-orchestrated and acts as a sequential defense mechanism for any infection related to
oral disease. Chronic periodontitis is a disease of a microbial challenge to symbiosis and homeostasis. Periodontal surgery
is the most promising cure with repair process during periodontal regeneration. It has an encouraging outcome in terms of
early recovery biomarkers.
Objective Saliva of periodontal surgery subjects with the chronic periodontitis have been evaluated by
1
H NMR spectros-
copy in search of possible early metabolic diferences that could be obtained in order to see the eradication of disease which
favours the symbiotic condition.
Method The study employed
1
H NMR spectroscopy on 176 human saliva samples in search of distinctive diferences and
their spectral data were further subjected to multivariate and quantitative analysis.
Result The
1
H NMR study of periodontal surgery samples shows clear demarcation and profound metabolic diferences
when compared with the diseased condition. Several metabolites such as lactate, ethanol, succinate, and glutamate were
found to be of higher signifcance in periodontal surgery in contrast to chronic periodontitis subjects. The PLS-DA model
of the studied group resulted in R
2
of 0.83 and Q
2
of 0.70.
Conclusion Signifcant metabolites could be considered as early repair markers for chronic periodontitis disease as they are
being restored to achieve symbiosis. The study, therefore, concluded the early recovery process of the diseased subjects with
the restoration of possible metabolomic profle similar to the healthy controls.
Graphic abstract
Electronic supplementary material The online version of this
article (https://doi.org/10.1007/s11306-019-1593-3) contains
supplementary material, which is available to authorized users.
Extended author information available on the last page of the article