Submit Manuscript | http://medcraveonline.com Introductıon Vegetable cultivation is carried out in almost every both greenhouse and feld condition and constitutes 95% of the total greenhouse cultivation in our country. Generally, white fies, aphids and red spiders cause signifcant loss of crops in vegetable cultivation. Myzus persicae Sulzer (Hem.: Aphididae) which is harmful in vegetables. M. persicae usually feeds on fresh shoots of the plant and weakens the growth of the plant. It is also the vector of some virus diseases. 1 Chemical pesticides are used to control both pests and consumption of chemical pesticides is increasing day by day. Chemical pesticides used intensively and unconsciously especially in greenhouse vegetable growing caused residues in the product, resistance to pests and constitutes a great risk for human health, nature and environment. It also creates an important problem in vegetable exports. Due to the damage of chemical pesticides, researchers have to search for alternative methods to control harmful pests. Based on these researchers have focused on different control methods. Biopesticides are derived from such natural materials as animals, plants, bacteria. Most work on this subject has been carried out on plants. Plants have been used in pest control since ancient times. The ancient Romans with the use of insecticide-containing olive oil and hellebore plants 2 conducted the frst application. The use of biopesticides in the feld of both conventional and organic farming, integrated crops and good agricultural practices is of great importance due to their advantages. Plant-specifc pesticides obtained from plants do not have additional toxic substances due to their presence in nature. Biopesticides do not cause soil and water pollution as decomposed in a short time and do not form residue on the products. 3 The subject that host selection by phytophag pests plays an important role in secondary plant compounds has increased the interest in nutritional inhibitory studies of pests. Hundreds of plant compounds have feeding inhibitory effects. 4 Warthen & Morgan 5 listed more than 400 plants with feeding inhibitory properties. In this area, the most research were carried out on Azadirachta indica (A. Juss). 6 In recent, there are commercial product developed from A. indica such as Margosan-O, Azatin, Bioneem, Neemguard, Neem Azal T / S. Later studies, the active ingredient is capsicin, Albanian pepper extract were developed into a preparation. This extract has been used to control so many pests such as aphids, thrips and red spiders, white fies in greenhouse and feld conditions. 3 There are many studies have been carried out in search of alternative to chemical pesticides In Turkey and studies are still going on. However, no studies have been conducted on the formulation of plant extracts. There is no biopesticide recommended to the application and there is a problem in this regard. Especially in organic agriculture, there is a great need for environmentally friendly and residue-free preparations. In Turkey, there are no biopesticides available for each product, and all recommended biopesticides are of international origin. Due to its rich plant fora studies carried out with plant extracts are important in Turkey. Biopesticides derived from local sources and plant origin substances are important to organic agriculture, integrated crops, good agricultural practices and national economy. Therefore, this study was conducted to prepare the formulation of two plant extracts (A. sallivum and M. azederach) and determine their effect on M. persicae. Material and method Plants Allium sativum L. (Amaryllidaceae), was provided from Kastamonu and Melia azedarach L. (Meliaceae) was collected from Adana provinces in 2016. Fruits of both plants were used to prepare extract. J Bacteriol Mycol Open Access. 2020;8(3):72‒76. 72 ©2020 ERDOGAN et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and build upon your work non-commercially. Formulation of Allium sativum L. and Melia azedarach L. plant extracts and their effects on Myzus persicae Sulzer, 1776 (Hemiptera: Aphididae) Volume 8 Issue 3 - 2020 Pervin ERDOGAN, 1 Pelin AKSU, 2 Gamze ESİN KILINC, 2 Murat KAHYAOGLU, 3 Numan E BABAROGLU 2 1 Department of Plant Protection, Sivas Science and Technology University, Turkey 2 Plant Protection Central Research Institute, Turkey 3 Agriculture and Chemical industry Trade Corporation, Turkey Correspondence: Pervin ERDOGAN, Department of Plant Protection, Faculty of Agricultural Sciences and Technology, Sivas Science and Technology University, Turkey, Tel +90346 219 13 98, Fax +90 346 219 16 78, Email Received: August 05, 2020 | Published: August 19, 2020 Abstract Myzus persicae Sulzer, 1776 (Hem.: Aphididae) the most important pests of vegetable cultivated in the world. Pesticides are used extensively to control this pest. Intensive use of chemical pesticides to control pests caused various side effects such as residues in the product, pests’ resistance and push a great risk for human health, nature and environment. This study was undertaken to provide an alternative to chemical pesticides. For this purpose, the extract of Allium sativum L. (Liliaceae) and Melia azedarach L. (Meliaceae) were prepared. Then formulation studies of these extracts were carried out with several inert ingredients. Obtained preparations were subjected to quality control tests in the laboratory. As a result of these tests, preparations which were found successfully were separated/ chosen for effectiveness studies on M. persicae. According to the results of laboratory studies, the highest dose found to be effective and theirs two upper doses (10, 15, 20ml/L) were taken to examine effect on M. persicae at the greenhouse conditions. The trial was established as randomized block design and 5 times in greenhouse. Commercial product Neem Azal T/S was used as positive control. The results of the greenhouse trials revealed that the insecticidal effect of M. azederach and A. sativum plants extract were 90.33% and 79.50% respectively. Also, it was determined that the insecticidal effect of M. azederach plant extract and Neem Azal T/S were 94.38% and 92.81 respectively. Keywords: green peach aphid, plant extracts, formulations, insecticidal effect Journal of Bacteriology & Mycology: Open Access Research Article Open Access