International Journal of the Computer, the Internet and Management Vol.27 No.2 (May-August, 2019) pp. 43-51 43 Molecular Marker Discovery for Ovarian Maturation Level of the Black Tiger Shrimp from Microarray Data Using Genetic Algorithm Supoj Hengpraprohm 1 , Suwimol Jungjit 2 , Kairung Hengpraprohm 3 , and Dech Thammasiri 4 1, 3 Program in Data Science, Faculty of Science and Technology, 4 Program in Business Computer, Faculty of Management Science, Nakhon Pathom Rajabhat University, Thailand 2 Department of Computer and Information Technology, Faculty of Science, Thaksin University, Thailand 1 supojn@yahoo.com 2 suwimol@tsu.ac.th 3 kairung2011.heng@gmail.com 4 dechit@msn.com Abstract - This paper proposes an embedded-based gene selection method in the black tiger shrimp microarray dataset using Genetic Algorithm (GA). Moreover, we analyze the effect of selected genes for ovarian maturation level in the term of biological perspective. We run experiment on 6 black tiger shrimp microarray datasets which were publically available. First, we run GA to find the optimized individual length. Next, the GA-based predictive model is run to predict the Gonadosomatic Index (GSI) of ovarian maturation level in black tiger shrimp. Finally, the most frequently selected genes from GA are observed and analyzed. The experimental results show that the optimized individual length for GA is 4 and the selected informative genes are from heart and gill tissues of black tiger shrimp. Keywords - Gene Selection, Microarray Data, Genetic Algorithms, Black Tiger Shrimp, Ovarian Maturation Level I. INTRODUCTION Black tiger shrimp was the major economic product in Thailand. However, the shrimp industry has experienced many problems such as slow growth, epidemics and incomplete breeding of shrimp brood stock from ponds. The shrimp‟s industry is greatly reduced [1]. Especially the non-reproductive problems of the black tiger shrimp are necessary to capture. The results of using too much of the wild shrimp are that cannot control the quality of the shrimp. This problem obstructs progress in the development of animal species and livestock industry. Eyestalk Ablation is a popular method to stimulate the development of ovaries in black tiger shrimp. Although the cuttings from shrimp ponds can stimulate the development of ovaries, the shrimp is still low quality for the industry. Therefore, understanding the effect of eye shear on the reproduction of black tiger prawn is very important. In [2] authors studies on the expression of genes that result from eye removal comparing of black tiger prawn from pond before and after cutting at different time.