Journal of Basic & Applied Sciences, 2016, 12, 185-191 185 ISSN: 1814-8085 / E-ISSN: 1927-5129/16 © 2016 Lifescience Global Inclusion of Blend of L-lysine, DL-Methionine and Vitamins D3, E in Diets for Broiler; Effects on Growth Performance, Feed Intake and Conversion and Water Intake Rani Abro 1,* , Abdul Ghaffar 1 , Shahid Hussain Abro 2 , Hakimzadi Wagan 3 , Ahmed Nawaz Tunio 4 , Jameel Ahmed Gandahi 5 , Pershotum Khatri 6 , Huma Rizwana 7 , Riaz Ahmed Leghari 2 , Bakhtawar Wagan 8 and Mohsan Ullah Goraya 9 1 Department of Animal Nutrition, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 2 Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 3 Department of Agricultural Economics, Faculty of Social Sciences, Sindh Agriculture University Tandojam, Pakistan 4 Department of Surgery & Obstetrics, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 5 Department of Anatomy and Histology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 6 Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam 7 Department of Livestock Management, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Pakistan 8 Department of Farm Structure, Sindh Agriculture University Tandojam, Pakistan 9 Life Science College, Fujian Agriculture and Forestry University, Fuzhou, China Abstract: The blend comprising of L-lysine, DL-methionine and vitamins D3, E in diets evaluated for growth performance, water intake, feed consumption and conversion ratio and live body weight in broiler was investigated. The study was performed on 360 day old broiler chickens and divided into four groups i.e. A (control), B, C and D (30 chickens in each group) randomly. The birds were fed blend in the diets; group B (40gm / 50 kg feed), group C (80 gm / 50 kg feed) and group D (120 gm / 50 kg feed). The growth parameters of broilers were significant (P<0.05) high in the diet group D in comparison to C, B and A groups respectively. The broiler in group D consumed more feed (4303.20 g/bird) followed by the broiler in C, B and A groups respectively. The significant difference (P<0.05) was determined in water intake of broilers among the groups. The broiler in group D was gained more live body weight (2301.60 g/bird) followed by the broilers in C (2094.50), B (2009.00) and A (1922.20 g/bird). The broiler in group D resulted superior feed conversion efficiency (1.87); followed by the broilers in group C (1.97). The broiler in groups B and A (control) resulted in lowest feed conversion efficiency (1.99 and 2.00) respectively. Overall, the supplementation of the blend in feed 120gm / 50 kg significantly influence on the feed consumption, water intake, live body weight and growth performance in broiler chickens. Keywords: Lysine, DL-methionine, vitamins, growth, feed, broiler. 1. INTRODUCTION Growth promoters are supplemented to poultry and livestock feed in order to efficiently metabolize and utilize feed for the better production and economic benefits. The various growth promoters such as vitamins, amino acids, antibiotics, herbal products and minerals are used in poultry feed [1]. The mechanism *Address correspondence to this author at the Department of Animal Nutrition, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam, Sindh, Pakistan; Tel: +92 307 2671526; E-mail: abrosau@yahoo.com of action of different growth promoters varies and mostly depends upon the nature. Generally, the positive actions are expressed through increasing appetite, improving efficiency of feed consumption and conversion, growth performance, enhancing vitality; maintain intestinal micro-flora and improve immunity and health in different animals [2, 3]. DL-methionine, L-lysine are limiting amino acids are commonly used in poultry diet in order to replace expansive natural protein sources [4, 5]. These amino acids are needed for the protein synthesis in avian