S172 Poster presentations of UC compared to those of CD (p<0.05). The positive correlation between Mayo score and IL-1R1 expression in intestinal mucosa was observed (r=0.68, p=0.028). The expression of IL-1β, IL-6, IL-8, and oncostatin M (OSM) was higher in steroid-dependent UC patients, and the primary non-responders to anti-TNFα therapies had higher expressions of IL-7 in colonic mucosa than the secondary non-responders in UC. Conclusion: Our results suggest that UC exhibits different cytokine patterns in the intestinal mucosa from CD as reported previously. Exploration of cytokine expressions in the intestinal mucosa may lead to predicting refractory IBD. The identifcation of molecules that can predict therapeutic effectiveness helps to optimize IBD treatment in the future. P067 The effect of a probiotic mix on mucosal healing and fibrotic responses in healthy colonic subepithelial myofibroblasts. G. Tarapatzi 1 , E. Filidou* 1 , L. Kandilogiannakis 1 , K. Arvanitidis 1 , S. Vradelis 2 , K. Kotzampassi 3 , G. Kolios 1 1 Democritus University of Thrace, Laboratory of Pharmacology- Faculty of Medicine, Alexandroupolis, Greece, 2 Democritus University of Thrace, Second Department of Internal Medicine- University Hospital of Alexandroupolis, Alexandroupolis, Greece, 3 Aristotle University of Thessaloniki, Department of Surgery- AHEPA Hospital, Thessaloniki, Greece Background: Various probiotic strains play a positive role in Infammatory Bowel Disease and might support mucosal healing. Colonic Subepithelial Myofbroblasts (SEMFs) have a pivotal role in mucosal healing and fbrosis, by migrating to the trauma region and secreting collagen. Our study aimed to investigate whether dif- ferent strains of probiotics can affect the migration capacity and the fbrotic phenotype of SEMFs isolated from healthy individuals. Methods: The probiotic mix of Lactobacillus acidophilus, Lactobacillus plantarum, Bifdobacterium lactis and Saccharomyces boulardii was reconstituted in SEMFs culture medium, they were identifed by Gram staining and their viability was assessed by Trypan Blue staining. Primary SEMFs were isolated from colonic biopsies from healthy individuals, and stimulated with 10 2 , 10 4 or 10 8 cfu/ml of the mix for 6h or 48h; in 6h total RNA was collected and mRNA expression of collagen type I, III, fbronectin, tissue fac- tor (TF) and α-SMA was measured by quantitative PCR, and in 48h Abstract P065 Downloaded from https://academic.oup.com/ecco-jcc/article/15/Supplement_1/S172/6286076 by guest on 19 January 2024