_____________________________________________________________________________________________________ *Corresponding author: E-mail: g.benhusein@uot.edu.ly; British Journal of Pharmaceutical Research 13(5): 1-10, 2016, Article no.BJPR.28796 ISSN: 2231-2919, NLM ID: 101631759 SCIENCEDOMAIN international www.sciencedomain.org In vitro Genotoxic Effect of Arsenical Compounds in HepG2 Liver Cells Ghazalla Benhusein 1* , Elaine Mutch 2 , Jamal Almezogi 3 and Faith Williams 2 1 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Tripoli University, P.O.Box 13645, Tripoli, Libya. 2 Toxicology Unit, Department of Environmental Medicine, University of Newcastle upon Tyne, 4th Floor Devonshire Building, NE1 7RU, United Kingdom. 3 Department of Phamacognocy, Faculty of Pharmacy, Tripoli University, P.O.Box 13645, Tripoli, Libya. Authors’ contributions This work was carried out in collaboration between all authors. Authors FW and EM designed the study, wrote the protocol. Author GB wrote the first draft of the manuscript, managed the literature searches, analyses of the study and managed the experimental process. Authors GB and JA read and approved the final manuscript. Article Information DOI: 10.9734/BJPR/2016/28796 Editor(s): (1) Jinyong Peng, College of Pharmacy, Dalian Medical University, Dalian, China. Reviewers: (1) Wissam Zam, Al-Andalus University for Medical Sciences, Syria. (2) Chi-Ming Liu, Tzu Hui Institute of Technology, Taiwan. (3) Bousserouel Souad, University of Strasbourg, France. (4) Weiting Wang, Tianjin Institute of Pharmaceutical Research, China. Complete Peer review History: http://www.sciencedomain.org/review-history/16752 Received 5 th August 2016 Accepted 4 th October 2016 Published 2 nd November 2016 ABSTRACT Aims: The aim of this study was to determine the genotoxic effect in HepG2 cells of the four arsenic compounds by measuring DNA damage, depletion of cellular glutathione (GSH) as a measure of oxidative stress as well as antioxidant agent and apoptotic and necrotic events. Study Design: Arsenic is an environmental chemical of toxicological concern today since it has been shown to be a human genotoxin and carcinogen. This project investigated four forms of inorganic arsenic: arsenate (As +5 ), sodium arsenite (As +3 ), arsenic trioxide (As 2 O 3 ) and dimethyl arsenic acid (DMA +5 ); a major metabolite of arsenate. Methodology: HepG2 cells (1.5x10 5 /ml) treated with the four arsenics (10 μM) for 24hr. Harvested Original Research Article