1 3
Arch Gynecol Obstet
DOI 10.1007/s00404-014-3275-8
GENERAL GYNECOLOGY
The platelet activating factor acetyl hydrolase, oxidized
low-density lipoprotein, paraoxonase 1 and arylesterase levels
in treated and untreated patients with polycystic ovary syndrome
Ayse Carlioglu · Ikbal Kaygusuz · Feridun Karakurt · Ilknur Inegol Gumus ·
Aysel Uysal · Benan Kasapoglu · Ferah Armutcu · Sema Uysal ·
Esra Aktepe Keskin · Cemile Koca
Received: 15 November 2013 / Accepted: 28 April 2014
© Springer-Verlag Berlin Heidelberg 2014
and they were statistically significantly lower in patients
treated with metformin or Diane-35 than untreated PCOS
patients. In contrast, there were lower PON1 (not statisti-
cally significant) and ARE (statistically significant) levels
in untreated PCOS patients than the control group and they
significantly increased after metformin and Diane-35 treat-
ments. In PCOS patients serum PON1 levels for QQ, QR
and RR phenotypes were statistically significantly lower
than the control group.
Conclusion In patients with PCOS, proatherogenic
markers increase. The treatment of PCOS with metformin
or Diane-35 had positive effects on lipid profile, increased
PON1 level, which is a protector from atherosclerosis
and decreased the proatherogenic PAF-AH and ox-LDL
levels.
Keywords Polycystic ovary syndrome · Platelet
activating factor acetyl hydrolyze · Oxidized LDL ·
Paraoxonase1 · Paraoxonase1 phenotypes · Arylesterase
Abstract
Purpose To evaluate the platelet activating factor acetyl
hydrolyze (PAF-AH), oxidized low-density lipoprotein (ox-
LDL), paraoxonase 1 (PON1), arylesterase (ARE) levels
and the effects of metformin and Diane-35 (ethinyl oestra-
diol + cyproterone acetate) therapies on these parameters
and to determine the PON1 polymorphisms among PCOS
patients.
Methods Ninety patients with PCOS, age 30, and body
mass index-matched healthy controls were included in the
study. Patients were divided into three groups: metformin
treatment, Diane-35 treatment and no medication groups.
The treatment with metformin or Diane-35 was continued
for 6 months and all subjects were evaluated with clini-
cal and biochemical parameters 6 months later. One-way
Anova test, t test and non-parametric Mann–Whitney U
tests were used for statistical analysis.
Results PAF-AH and ox-LDL levels were statistically sig-
nificantly higher in untreated PCOS patients than controls,
A. Carlioglu
Department of Endocrinology, Regional Education and Research
Hospital, Erzurum, Turkey
I. Kaygusuz (*) · I. I. Gumus · E. A. Keskin
Department of Obstetrics and Gynecology, Turgut Ozal
University School of Medicine, Ciftlik Cad. No:57,
Emek, 06510 Ankara, Turkey
e-mail: ikbal_cekmen@yahoo.com
F. Karakurt
Department of Endocrinıology, Gazi University Medical School,
Ankara, Turkey
A. Uysal
Department of Obstetrics and Gynecology, Education
and Research Hospital, Antalya, Turkey
B. Kasapoglu
Department of Internal Medicine, Turgut Ozal University
Medical School, Ankara, Turkey
F. Armutcu
Department of Biochemistry, Turgut Ozal University Medical
School, Ankara, Turkey
S. Uysal
Department of Biochemistry, Numune Education and Research
Hospital, Ankara, Turkey
C. Koca
Department of Biochemistry, Atatürk Education and Research
Hospital, Ankara, Turkey