Vol. 13(29), pp. 658-666, November, 2019 DOI: 10.5897/AJMR2019.9166 Article Number: 82A588262276 ISSN: 1996-0808 Copyright ©2019 Author(s) retain the copyright of this article http://www.academicjournals.org/AJMR African Journal of Microbiology Research Full Length Research Paper Assessment of drugs pressure on Escherichia coli and Klebsiella spp. uropathogens in patients attending Abobo-Avocatier Hospital, North of Abidjan (Côte d’Ivoire) Innocent Allepo ABE 1,2 , Mathurin KOFFI 2* , Paulin Didier SOKOURI 1 , Bernardin Ahouty AHOUTY 1,2 , Martial Kassi N’DJETCHI 2 , Siriki SIMARO 2 , Edwige Abla SOKOURI 2 , Barkissa TRAORE 2 , Thomas Konan KONAN 2 , William YAVO 3 , Sanogo Abiba TIDOU 2 and Simon-Pierre N’GUETTA 1 1 Laboratoire de Génétique, UFR Biosciences, Université Félix Houphouët-Boigny, 22 BP 582 Abidjan 22, Côte d’Ivoire. 2 Unité de Recherche en Génétique et Epidémiologie Moléculaire (URGEM), Laboratoire de Biodiversité et Gestion durable des Ecosystèmes Tropicaux, UFR Environnement, Université Jean Lorougnon Guédé, BP 150 Daloa, Côte d’Ivoire. 3 Institut National de Santé Publique (INSP), 01 BPV 34 Abidjan-Côte d'Ivoire. Received 7 July, 2019; Accepted 9 October, 2019 The problem of antibiotic resistance of uropathogens appears in Abobo municipality to be worsening because of the overuse and misuse of antibiotics. This study aims to assess the impact of drugs pressure on uropathogenic Escherichia coli and Klebsiella spp. strains isolated from patients attending Abobo-Avocatier Hospital. The study was conducted in patients suffering from urinary tract infection. Urine samples of patients were collected; culture and antibiogram using the Kirby-Bauer disc diffusion method were performed. The overall prevalence was 31.1% with a significant difference between males and females (p = 0.01). The highest susceptible age group of patients to UTI was 21-45 years (33%). E. coli and Klebsiella spp were the predominant bacteria among isolated Gram negative. Up to 70% of the isolates of both uropathogens were resistant to Penicillins, Tetracyclines and Ampicillin-Sulbactam. Imipenem was the most active antibiotic on these uropathogens. Quinolones showed a better activity on Klebisiella spp. strains than those of E. coli. The high value of the Multiple Antibiotic Resistance Index and the rate of multi-resistance from this site suggest the need for continuous monitoring of antibiotic susceptibility profile of bacteria implicated in UTI prior to antibiotic prescription in order to ensure optimal and desired treatment. Key words: Urinary tract infections, uropathogens, multidrug resistance, Abidjan, Côte d’Ivoire. INTRODUCTION Urinary tract infection (UTI) is defined as a multiplication of microorganisms in the urinary tract with or without symptoms (Prakash and Saxena, 2013). This pathology usually starts by a bladder infection and can reach the kidneys to cause renal dysfunction or dissemination in the blood (Vejborg et al., 2011). Depending on the organ