Anal. Chem. Lett. 2022, 12, 266-282 DOI: 10.1080/22297928.2021.2021110 © 2022 Har Krishan Bhalla & Sons Binding and Photocleavage Studies of Ru (II) Polypyridyl Complexes with DNA: An In Silico and Antibacterial activity Navaneetha Nambigari 1,2 *, Aruna Kodipaka 1 , Ravi Kumar Vuradi 2 , Praveen Kumar Airva 3 and Satyanarayana Sirasani 2 * 1 Department of Chemistry, University College of Science, Osmania University, Saifabad, Hyderabad-500004, Telangana, India 2 Department of Chemistry, University College of Science, Osmania University, Tarnaka, Hyderabad-500007, Telangana, India 3 Department of Biotechnology, Sri Satya Sai University of Technology & Medical Sciences, Opp. Oilfed Plant, Bhopal- Indore Road, Sehore-66001, Madhya Pradesh, India * Corresponding Authors: nitha379@gmail.com (Navaneetha Nambigari) ssnsirasani@gmail.com (Satyanarayana Sirasani) Supplementary Information Experimental All the Reagents and solvents of analytical grade were used as received unless otherwise stated. 1, 10-Phenanthroline monohydrate (phen), 2,2’-bipyridine (bpy),4,4’-dimethyl- 2,2’-bipyridine (dmb), and 4,4’-dimethyl-1,10 orthophenonthroline (dmp) were procured from Merck. Calf thymus DNA (CT-DNA) was purchased from Aldrich, Super coiled pBR322 plasmid DNA (stored at 20ºC) from Fermentas Life Sciences and was utilized as it is. Agarose gel was bought from Genei. Ultrapure Milli-Q water (18.2mX) was used in all experiments. The binding afnity of metal complex for CT- DNA was calculated in tris (hydroxymethyl) amino methane, Tris–HCl bufer (5 m.mol Tris- HCl, 50 mM NaCl, pH 7.2). The DNA, free of protein was observed at an absorbance of 260 and 280 nm and a concentration of DNA per nucleotide at a molar extinction Coefcient of 6600 M -1 cm -1 was determined spectrophotometrically 1,2 . Stock solutions of DNA were kept at 40ºC and were put to use in less than four days. The stock solutions of concentrated metal complexes (calculated amount) were made in DMSO, and were further diluted to the requisite concentrations with complimenting bufers for every experiment. The UV-Vis spectra were recorded on Shimadzu UV-2600 spectrophotometer. For recording the luminescence spectral data for determining the binding constants, Cary Eclipse instrument serial number (MY12400004) Spectrofuorometer was employed. IR spectra were captured on a PerkinElmer 1605 Fourier transform IR spectrometer using KBr disks. 1 H and 13 C NMR spectra were recorded by using a Bruker 400-MHz spectrometer with dimethyl-d6 sulfoxide (DMSO-d6) as the solvent and tetramethylsilane as the internal standard at room temperature. Elemental microanalysis (C, H, and N) was performed by using PerkinElmer 240 elemental analyzer. Mass spectra were recorded with a Quattro LC triple quadrupole mass spectrometer fortifed with the Mass Lynx software program (Micro mass, Manchester, UK) 3 . i