Studying the Influence of Illumination Design in Quality Inspection on Vaccine Vials Production Line Using Computer Vision Sy Hieu Dau 1,2,* , Quang My Han Doan 1,2,* , Chiu Hy Ta 1,2 , Nguyen An Khang Le 1,2 , Nguyen Thanh Dat Khau 1,2 1 Department of Biomedical Engineering Physics, Faculty of Applied Science, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City, Vietnam. 2 Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City,Vietnam. dausyhieu@hcmut.edu.vn, han.doanquang@hcmut.edu.vn Abstract In the industrial context, there are key factors that directly affect the system’s efficiency. Higher demands for both quantity and quality in today’s market call for constant research and development of technologies for automating production and quality control. Machine vision is a solution to increase speed and accuracy in defect detection. However, applications from machine vision are only effective if there is good data input. This is the reason why a machine vision system, needs high-quality input images from a well-designed illumination system. These illumination systems are designed to highlight faults in products. Therefore, the images obtained will provide optimized data for easier image processing thus directly increase the processing speed, accuracy, and overall system performance. To achieve this goal, this paper presents a few approaches to enhance and optimize images by implements illumination techniques into a miniature model of pharmaceutical bottle assembly line using machine vision as the inspector block. In this paper, we will evaluate the critical needs of using customize illumination system for quality inspection on an assembly line. Keywords: Assembly line, Automatic Optical Inspection, Computer Vision, Image processing, Inspection, Illumination, LED lamp, Machine Vision, Optics, Vaccine. * Corresponding author Kalpa Publications in Engineering Volume 4, 2022, Pages 9–19 Proceedings of International Sym- posium on Applied Science 2021 T.T. Truong, T.N. Tran, T.N. Nguyen and Q.K. Le (eds.), ISAS 2021 (Kalpa Publications in Engineering, vol. 4), pp. 9–19