Open Journal of Medical Microbiology, 2021, 11, 32-46
https://www.scirp.org/journal/ojmm
ISSN Online: 2165-3380
ISSN Print: 2165-3372
DOI: 10.4236/ojmm.2021.111003 Mar. 11, 2021 32 Open Journal of Medical Microbiology
Prevalence, Species Distribution and Antifungal
Susceptibility Profile of Candida Species
Isolated from Bloodstream of Critical Care Unit
Patients in a Tertiary Care Hospital in Kenya
Danait Andemichael Solomon
1*
, Andrew K. Nyerere
1
, Alice Kanyua
2
, Caroline Wangari Ngugi
1
1
Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology, 62000-00200, Nairobi, Kenya
2
Nairobi Hospital, 30026-00100, Nairobi, Kenya
Abstract
The upsurge of candidemia in the past years has been an immense encum-
brance on public health and the number of deaths caused by candidemia par-
ticularly in critical care unit patients is devastating. Candida species harbor a
30% - 60% mortality rate and compared to stable people or those with less se-
rious illnesses, this ranges from 60% to 80% of those who are chronically ill
patients. Grounded on a recent report from a tertiary care hospital in Kenya
showing the emergence of previously unobserved species: Candida auris, this
study aimed to determine the prevalence, species distribution, and antifungal
susceptibility profile of candidemia in critical care unit patients of the hospit-
al. 378 Critical Care Unit patients were enrolled for the study from January
2019 to January 2020. Positive archived isolates were sub-cultured using Sa-
boraud Dextrose Agar. Candida species were identified utilizing API20C AUX
and Vitek-2. Antifungal susceptibility testing was conducted using the Liofil-
chem MIC Test strip. Out of 378 patients, thirty-one presented a positive
culture for Candida species. The prevalence of Candidemia was 8.2% with 9
(29.03%) Candida auris, 8 (25.81%) Candida albicans, 6 (19.35%) Candida
parapsilosis, 3 (9.68%) Candida famata, 3 (9.68%) Candida tropicalis, 1
(3.23%) Candida duobushaemolumonii, and 1 (3.23%) Candida lusitaniae. A
resistance pattern to Fluconazole was observed among Candida auris and
Candida parapsilosis, and resistance to Flucytosine was observed in Candida
tropicalis, whereas susceptible MIC values were obtained for the other drugs.
There is an increase in candidemia among critical care unit patients in the
health facility posing a public health challenge. Moreover, the onset of new
species Candida auris which is unprecedented in Kenya warrants enhanced
infection control, and the uniform resistance of Candida auris, Candida pa-
How to cite this paper: Solomon, D.A.,
Nyerere, A.K., Kanyua, A. and Ngugi, C.W.
(2021) Prevalence, Species Distribution and
Antifungal Susceptibility Profile of Candida
Species Isolated from Bloodstream of Crit-
ical Care Unit Patients in a Tertiary Care
Hospital in Kenya. Open Journal of Medi-
cal Microbiology, 11, 32-46.
https://doi.org/10.4236/ojmm.2021.111003
Received: January 30, 2021
Accepted: March 8, 2021
Published: March 11, 2021
Copyright © 2021 by author(s) and
Scientific Research Publishing Inc.
This work is licensed under the Creative
Commons Attribution International
License (CC BY 4.0).
http://creativecommons.org/licenses/by/4.0/
Open Access