Journal of Health Sciences and Innovative Research 2022;3(1):20-28 DOI: https://doi.org/10.31357/jhsir.v3i01.5968 © University of Sri Jayewardenepura Introduction Evidence-based practice (EBP) is defined as the integration of the best available external research findings with clinical expertise and patients’ preferences. Simply, when treating a patient, it is the physiotherapist’s responsibility to find the best available high-quality clinical research and combine them with their practice knowledge while Research Article Abstract Introduction: Implementing evidence-based practice (EBP) is beneficial for the patients as they can receive the best available treatment while improving the reputation of the profession. This study aimed to assess the knowledge, attitudes, and perceived barriers towards EBP among physiotherapists serving in Sri Lankan government hospitals. Method: A cross-sectional descriptive study was conducted using a convenient sampling method among 264 physiotherapists in thirty-nine government hospitals in Sri Lanka between 18 th November 2019 and 23 rd December 2019. A pre-tested, validated, and self- administered questionnaire with twenty-six items was categorized into four main parts. These included demographic data of the participants (seven items), their knowledge (six items), attitudes (five items), and perceived barriers towards EBP (eight items). The responses under attitudes and knowledge sections were scored using a 5-point Likert scale ranging from 0-4, with higher scores indicating favourable results. Based on the sum of marks obtained for the attitude part, subjects were categorized as; negative (0-7), neutral (8-15), and positive (16-24) attitudes. Based on the sum of the marks obtained for the knowledge part, subjects were categorized as; poor (0-6), average (7-13), and good (14-20) knowledge. Data analysis was done using the statistical software Minitab version 2018. Results: Among the participants, 1.5% had a poor level of knowledge, 22.7% had an average level of knowledge, and 75.8% had good knowledge regarding EBP. Attitudes toward EBP were positive among 87.9% of participants while 11.7% had neutral attitudes, and 0.4% had negative attitudes. The common perceived barriers to practice EBP were, lack of time, research skills, information resources, and interest towards EBP. Conclusion: A selected sample of government physiotherapists in Sri Lanka had positive attitudes and good knowledge towards EBP and are inclined to incorporate evidence into their clinical practice. Keywords: Evidence-based practice, Physiotherapists, Knowledge, Attitudes, Barriers, Implementation Knowledge, Attitudes, and Perceived Barriers Towards Evidence-based Practice Among Government Sector Physiotherapists in Sri Lanka Samarakoon S.M.C.S. 1* , Perera L.P.S.G. 1 , Pahalawattage N.J. 1 , Riswan A.J.M. 1 , Madhushani R.D. 1 , Perera L.P.U.I. 1 , Senadheera V.V. 1 , Banneheka S. 2 1 Department of Physiotherapy, Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka 2 Department of Basic Sciences, Faculty of Dental Sciences, University of Peradeniya, Peradeniya, Sri Lanka *Corresponding author: firstsewwandi@gmail.com Received: 03 September 2021; Accepted: 09 July 2022 How to cite this article: Samarakoon, S.M.C.S., Perera, L.P.S.G., Pahalawattage, N.J., Riswan, A.J.M., Madhushani, R.D., Perera, L.P.U.I., et.al. Knowledge, Attitudes, and Perceived Barriers Towards Evidence-based Practice Among Government Sector Physiotherapists in Sri Lanka, Journal of Health Sciences and Innovative Research, 2022;3(1):20-28.