tests. Identifying specific proteins with the potential to become a pre- ventive test should eventually lead to a reduction in morbidity and mor- tality of PC. The results of this work should lay the foundation that can guide future research. 296 Identifying Gaps in Elderly Fecal Incontinence Management Braden Barlean, Khalil Merali, Meagan Smith, Poplar Yang Johns Hopkins University OBJECTIVES/GOALS: Fecal incontinence is associated with increased caregiver strain, reduced patient dignity and diminished quality of life. A spectrum of incontinence exists, along with a paucity of available solutions for patients and their caregivers. This research aims to stratify this space and identify gaps within the existing sol- ution landscape. METHODS/STUDY POPULATION: To under- stand this problem, a literature review was performed with key search terms specific to fecal incontinence. These included, anal incontinence epidemiology, fecal incontinence in nursing homes, and incontinence management. To determine gaps within the existing solution landscape, key search terms related to existing sol- utions for fecal incontinence were also included. These included, fecal management systems, rectal incontinence therapies, and ano- rectal incontinence procedures.' To perform a population segmenta- tion, white papers, review articles, and cross-sectional studies were reviewed to break down the burden of incontinence in older adults living in nursing facilities and in the community. RESULTS/ ANTICIPATED RESULTS: Two unaddressed populations were identified, the first being independent adults over the age of forty, particularly women, who suffer from frequent, bothersome inconti- nence. These 1.2 million patients are active, living at home, and they restrict their daily activities due to incontinence. However, there are several durable and effective solutions for patients who have suffi- cient sphincter tone or who are surgical candidates. The second pop- ulation identified are caregiver dependent older adults residing in nursing facilities who suffer from severe incontinence. This popula- tion of 160,000 is affected more severely by consequences of fecal and are poorly served by solutions that are largely absorptive such as dia- pers and pads. DISCUSSION/SIGNIFICANCE: Although two pop- ulations were identified, caregiver dependent older adults residing in nursing homes were identified to have a significant unmet need within incontinence care. Current solutions are onerous and tran- sient, preventing ease and duration for use by caregivers and nurses. 297 Identifying Opportunities and Challenges for Translational Informatics Approaches to Real-World Data: A Diabetes Case Study Sejal Mistry, Ramkiran Gouripeddi, Julio C. Facelli University of Utah OBJECTIVES/GOALS: Diabetes is a group of chronic metabolic dis- eases and significant gaps remain in our understanding of disease eti- ology, treatment regimens, and diabetes-related complications. The objective of study is to demonstrate how informatics techniques can leverage real-world data for diabetes research and identify barriers for implementation. METHODS/STUDY POPULATION: We evalu- ated informatics applications of real-world data in diabetes research conducted by the Facelli Research Group. The types of real-world data were categorized into clinical records, diabetes-related repositories, wearable sensors, and other data sources. Translational informatics applications were characterized into thematic groups of 1.) use of elec- tronic health records, registries, and claims and other data sources to generate real-world evidence, 2.) evolution of novel methods to accel- erate generation and use of real-world data, and 3.) infrastructure to support the generation and use of real-world data in translational sci- ence. A literature review is being conducted to identify additional articles meeting these themes focused on diabetes research. RESULTS/ANTICIPATED RESULTS: 6 research projects were included for analysis. The diabetes-focus spanned type 1 diabetes, type 2 diabetes, and general diabetes mellitus. Informatics methods included machine learning and data mining while real-world data sources included electronic medical records, the Environmental Determinants of Diabetes in the Young (TEDDY) study, continuous glucose monitors, and the U.S. Environmental Protection Agency (EPA) air pollution monitors. Overall, computability of real-world data, linkage of medical concepts to standardized terminologies, vol- ume of data, and adoption of novel artificial intelligence methods were major determinants of successful implementation. Future work will systematically evaluate informatics applications of real-world data in diabetes from the academic community at large. DISCUSSION/ SIGNIFICANCE: Translational informatics approaches are poised to leverage real-world data and better understand diabetes etiology, treat- ment regimens, and diabetes-related complications. By understanding barriers and opportunities for informatics methods, we can expedite translational applications in diabetes research. 298 Identifying vaginal microbiome profiles that influence tenofovir distribution in the female genital tract* Alyssa M. Lantz, Christopher Staley, Melanie R. Nicol University of Minnesota OBJECTIVES/GOALS: An ex-vivo tissue model has been developed to predict target concentrations of tenofovir diphosphate (TFVdp; active metabolite of tenofovir) but has not been utilized to see how vaginal dysbiosis affects TFVdp/dATP exposure in female genital tract (FGT). My central hypothesis is that presence of specific anaerobic bac- teria will increase dATP in FGT. METHODS/STUDY POPULATION: De-identified HIV-negative cervical tissues from women undergoing gynecological surgeries will be procured and a punch biopsy will be used to create explants. TFVdp/dATP concentrations were both tested in both aerobic and anaerobic conditions after a 24-hour incubation in tenofovir (TFV) to determine any changes between conditions. TFVdp/dATP in cervical tissue was be measured using LC-MS. Next, media and explants were collected at baseline to characterize donor microbiome for 6 donors. 16S microbiome sequencing was per- formed on extracted DNA to obtain the relative abundances of each bacteria species present. To test changes in dATP/TFVdp due to the microbiome, explants will be incubated in TFV for 24 hours with Prevotella and Dialister to specifically see how microbiomes dominated by these taxa affect dATP. RESULTS/ANTICIPATED RESULTS: There was no significant difference in TFVdp formation between aero- bic and anaerobic conditions after a 24-hour tenofovir incubation (p = 0.2) for 8 donors. dATP was not quantifiable at 24 hours in explants, so explants are being collected before 24hrs during a TFV incubation to determine how quickly dATP depletes after collection. We were able to characterize the donor microbiome in media and tissue at baseline and 24hrs which had inter variability. We did not see any presence of Prevotella or Dialister in any donors. We are working on characterizing JCTS 2023 Abstract Supplement 89 https://doi.org/10.1017/cts.2023.351 Published online by Cambridge University Press