Citation: Abeer Abdelrazig Osman, Hadia Abass Eltaib, Ghanem Mohammed Mahjaf, Tibyan Abd Almajed Altaher, Wafa Bashir Haj Ahmed, Mosab Nouraldein Mohammed Hamad (2023). Detection of Bacterial Contaminants from Operating Theatres at Hospitals in Shendi City, Sudan. SAR J Pathol Microbiol, 4(1), 7-10. 7 SAR Journal of Pathology and Microbiology Abbreviated Key Title: SAR J Pathol Microbiol Home page: https://sarpublication.com/journal/sarjpm/home DOI: 10.36346/sarjpm.2023.v04i01.002 ISSN 2707-7756 (P) ISSN 2709-6890 (O) Detection of Bacterial Contaminants from Operating Theatres at Hospitals in Shendi City, Sudan Abeer Abdelrazig Osman 1 , Hadia Abass Eltaib 1 , Ghanem Mohammed Mahjaf 1 , Tibyan Abd Almajed Altaher 2 , Wafa Bashir Haj Ahmed 3 , Mosab Nouraldein Mohammed Hamad 4* 1 Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan 2 Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Shendi University, Sudan 3 Department of Haematology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan 4 Assistant professor, Microbiology department, Faculty of Medicine, Elsheikh Abdallah Elbadri University, Sudan *Corresponding Author: Mosab Nouraldein Mohammed Hamad Assistant professor, Microbiology department, Faculty of Medicine, Elsheikh Abdallah Elbadri University, Sudan Article History: | Received: 16.01.2023 | Accepted: 23.02.2023 | Published: 25.02.2023 | Abstract: Background: Operating room contamination is recognized as one of the most common life-threatening microbial contaminations in hospital environments, especially operating rooms and other specialty units, and is an ever- increasing cause of nosocomial infections. Objective: The purpose of this study was to isolate and identify bacterial contaminants in Shendi hospital operating room. Between July-September 2021. Materials and Methods: Sixty samples were collected from various locations in the operating room and all isolated bacteria were identified. The study isolated five types of bacteria from the Shendi Hospital operating room. Results: The results showed that Micrococcus was the most common bacterial contaminant isolated from the operating room. Leteus 21 (32.3%) Staphylococci. Epidermis 16 (24.6%), Staphylococci aureus 11 (16.9%), Bacillus sp. 9 (13.8%), Bacillus cereus 8 (12.3%)) and the lowest contaminants were isolated from soil 15 (23%), and focus lamp 5 (7.7%). This study may point to the fact that the Shendi Hospital operating room had bacterial contamination that could lead to postoperative wound infections (SSI). Reasons for contamination may be due to excessive attendance, personnel movement, and ineffective sterilization and disinfection procedures. Conclusions: Bacterial contamination was highest in operating room beds, followed by carts, floors, and focused lambs. Micrococcus spp, S. epidermidis, S. aureus, and Bacillus species have proven to be the most serious contaminants in the operating room, and are a dangerous cause of hospital-acquired infections, killing patients and hospital staff threatening. This may indicate that the sterilization method is not efficient enough, putting the patient at risk for postoperative infection. Some organisms were resistant to Gentamicin and highly sensitive to Imipenem, Ciprofloxacin, and Ceftriaxone. Keywords: Operating Room, Nosocomial Infections, Leteus, Shendi, Sudan. Copyright © 2023 The Author(s): This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY-NC 4.0) which permits unrestricted use, distribution, and reproduction in any medium for non-commercial use provided the original author and source are credited. INTRODUCTION Nosocomial infections (also known as nosocomial/acquired infections) are infections that occur while the patient is hospitalized or in other clinical settings that were not present at the time of admission. The hospital environment is a potential reservoir for bacterial pathogens as it houses both patients with a variety of pathogenic organisms and large numbers of susceptible individuals with compromised immune systems. The increasing frequency of bacterial pathogens in the hospital environment is associated with an increasing background of different types of nosocomial infections [1]. Of particular importance for nosocomial infections are bacterial pathogens that survive for long periods in the hospital environment and resist disinfection [1]. Bacterial pathogens isolated from hospital environments are also known to develop resistance to some antimicrobial agents. The emergence of multidrug-resistant bacteria in hospitals has made the treatment of nosocomial infections difficult. Despite advances in modern medicine, nosocomial infections Original Research Article